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PMID: 12930902 Published · ppublish English Journal Article

The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 18 ·2003-09-02 ·Pages 10237-42

Bermudez VP, Maniwa Y, Tappin I, Ozato K, Yokomori K, Hurwitz J

Abstract

The linkage of sister chromatids after DNA replication ensures the faithful inheritance of chromosomes by daughter cells. In budding yeast, the establishment of sister chromatid cohesion requires Ctf8, Dcc1, and Ctf18, a homologue of the p140 subunit of the replication factor C (RFC). In this report we demonstrate that in 293T cells, Flag-tagged Ctf18 forms a seven-subunit cohesion-RFC complex comprised of Ctf18, Dcc1, Ctf8, RFCp40, RFCp38, RFCp37, and RFCp36 (Ctf18-RFC). We demonstrate that a stoichiometric heteroheptameric Ctf18-RFC complex can be assembled by coexpressing the seven proteins in baculovirus-infected insect cells. In addition, the two other stable subcomplexes were formed, which include a pentameric complex comprised of Ctf18, RFCp40, RFCp38, RFCp37, and RFCp36 and a dimeric Dcc1-Ctf8. Both the five- and seven-subunit Ctf18-RFC complexes bind to single-stranded and primed DNAs and possess weak ATPase activity that is stimulated by the addition of primed DNA and proliferating cell nuclear antigen (PCNA). These complexes catalyzed the ATP-dependent loading of PCNA onto primed and gapped DNA but not onto double-stranded nicked or single-stranded circular DNAs. Consistent with these observations, both Ctf18-RFC complexes substituted for the replicative RFC in the PCNA-dependent DNA polymerase delta-catalyzed DNA replication reaction. These results support a model in which sister chromatid cohesion is linked to DNA replication.

MeSH Terms
ATPases Associated with Diverse Cellular Activities Adenosine Triphosphatases/metabolism Carrier Proteins/physiology Cell Line Chromatids/physiology DNA/metabolism DNA Polymerase III/metabolism DNA Replication DNA-Binding Proteins/physiology Humans Nuclear Proteins/physiology Proliferating Cell Nuclear Antigen/metabolism Replication Protein C
Chemicals
CHTF18 protein, human CHTF8 protein, human Carrier Proteins DNA-Binding Proteins DSCC1 protein, human Nuclear Proteins Proliferating Cell Nuclear Antigen DNA DNA Polymerase III Adenosine Triphosphatases ATPases Associated with Diverse Cellular Activities Replication Protein C
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bermudez Vladimir P
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.
Maniwa Yoshimasa
Tappin Inger
Ozato Keiko
Yokomori Kyoko
Hurwitz Jerard
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1434308100
Published
2003-09-02
Epub
2003-00-20
Pages
10237-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC193545
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.1434308100 PMC Full Text View journal details
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