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PMID: 12958213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phagocytosis by neutrophils induces an amino acid deprivation response in Saccharomyces cerevisiae and Candida albicans.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 19 ·2003-09-16 ·Pages 11007-12

Rubin-Bejerano I, Fraser I, Grisafi P, Fink GR

Abstract

The transcriptional profiles of yeast cells that have been phagocytosed by either human neutrophils or monocytes were compared by using whole genome arrays. After phagocytosis by neutrophils, both Saccharomyces cerevisiae and Candida albicans respond by inducing genes of the methionine and arginine pathways. Neither of these pathways is induced upon phagocytosis by monocytes. Both fungi show a similar induction of these pathways when transferred from amino acid-rich medium to amino acid-deficient medium. These data suggest that the internal phagosome of the neutrophil is an amino acid-deficient environment.

MeSH Terms
Amino Acids/metabolism Candida albicans/metabolism Cell Cycle Hydrogen Peroxide/pharmacology Neutrophils/immunology,physiology Oxidative Stress Phagocytosis Saccharomyces cerevisiae/metabolism Transcription, Genetic/physiology
Chemicals
Amino Acids Hydrogen Peroxide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rubin-Bejerano Ifat
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA.
Fraser Iain
Grisafi Paula
Fink Gerald R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1834481100
Published
2003-09-16
Epub
2003-00-04
Pages
11007-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC196917
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035010 · United States
NIGMS NIH HHS · R01 GM040266 · United States
NIGMS NIH HHS · GM 40266 · United States
NIGMS NIH HHS · R01 GM35010 · United States
External Links
PubMed source DOI: 10.1073/pnas.1834481100 PMC Full Text View journal details
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