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PMID: 12958237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ribosomal DNA sequencing for identification of aerobic gram-positive rods in the clinical laboratory (an 18-month evaluation).

Journal of clinical microbiology ·Vol. 41 ·No. 9 ·2003-09-00 ·Pages 4134-40

Bosshard PP, Abels S, Zbinden R, Böttger EC, Altwegg M

Abstract

We have evaluated over a period of 18 months the use of 16S ribosomal DNA (rDNA) sequence analysis as a means of identifying aerobic gram-positive rods in the clinical laboratory. Two collections of strains were studied: (i) 37 clinical strains of gram-positive rods well identified by phenotypic tests, and (ii) 136 clinical isolates difficult to identify by standard microbiological investigations, i.e., identification at the species level was impossible. Results of molecular analyses were compared with those of conventional phenotypic identification procedures. Good overall agreement between phenotypic and molecular identification procedures was found for the collection of 37 clinical strains well identified by conventional means. For the 136 clinical strains which were difficult to identify by standard microbiological investigations, phenotypic characterization identified 71 of 136 (52.2%) isolates at the genus level; 65 of 136 (47.8%) isolates could not be discriminated at any taxonomic level. In comparison, 16S rDNA sequencing identified 89 of 136 (65.4%) isolates at the species level, 43 of 136 (31.6%) isolates at the genus level, and 4 of 136 (2.9%) isolates at the family level. We conclude that (i) rDNA sequencing is an effective means for the identification of aerobic gram-positive rods which are difficult to identify by conventional techniques, and (ii) molecular identification procedures are not required for isolates well identified by phenotypic investigations.

MeSH Terms
Bacteria, Aerobic/genetics,isolation & purification DNA, Bacterial/chemistry DNA, Ribosomal/chemistry Gram-Positive Rods/genetics,isolation & purification Humans Phenotype Prospective Studies RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Chemicals
DNA, Bacterial DNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bosshard P P
Institute of Medical Microbiology, University of Zürich, 8028 Zürich, Switzerland.
Abels S
Zbinden R
Böttger E C
Altwegg M
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
2003-09-00
Pages
4134-40
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC193817
Subset
IM
Analysis Services
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