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PMID: 1309613 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Viral proteins associated with the Epstein-Barr virus transactivator, ZEBRA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 1 ·1992-01-01 ·Pages 378-82

Katz DA, Baumann RP, Sun R, Kolman JL, Taylor N, Miller G

Abstract

The BamHI Z Epstein-Barr replication activator (ZEBRA) mediates disruption of latency and induction of Epstein-Barr virus (EBV) early gene expression in latently infected lymphocytes. Polyclonal rabbit sera raised against ZEBRA were used to immunoprecipitate ZEBRA-associated proteins (ZAPs). ZAPs of 19, 21, 23, and 42 kDa were coimmunoprecipitated with ZEBRA from extracts of EBV-producing lymphoid cell lines. ZAPs were not recognized directly by the rabbit sera, but they were antigenic for EBV+ human sera. Immunoprecipitation of ZAPs by ZEBRA-specific antisera required the presence of ZEBRA. ZAPs were not coprecipitated with ZEBRA from mouse cells expressing only ZEBRA, from Raji (a cell line in which EBV is unable to complete lytic replication), or from cells treated with inhibitors of viral DNA synthesis. Thus, ZAPs are late EBV-encoded proteins. ZEBRA and ZAPs colocalized to a salt-insoluble nuclear fraction, and both were found extracellularly in crude preparations of virions. ZAPs might function to affect the cellular localization of ZEBRA, to alter its capacity to transactivate, or to influence its target gene specificity.

MeSH Terms
Cell Line DNA-Binding Proteins/immunology,metabolism Herpesvirus 4, Human/genetics,growth & development Humans Macromolecular Substances Molecular Weight Nuclear Proteins/chemistry,metabolism Precipitin Tests Protein Binding Trans-Activators/metabolism Viral Proteins/chemistry,metabolism Virus Replication
Chemicals
BZLF1 protein, Herpesvirus 4, Human DNA-Binding Proteins Macromolecular Substances Nuclear Proteins Trans-Activators Viral Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Katz D A
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06510.
Baumann R P
Sun R
Kolman J L
Taylor N
Miller G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.1.378
Published
1992-01-01
Pages
378-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48240
Subset
IM
Grants
NIAID NIH HHS · AI22959 · United States
NCI NIH HHS · CA12055 · United States
NCI NIH HHS · CA16038 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.1.378 PMC Full Text View journal details
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