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PMID: 2849118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genome rearrangements activate the Epstein-Barr virus gene whose product disrupts latency.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 24 ·1988-12-00 ·Pages 9801-5

Rooney C, Taylor N, Countryman J, Jenson H, Kolman J, Miller G

Abstract

A defective Epstein-Barr virus (EBV) containing a deleted and rearranged genome (het DNA) causes latent EBV to replicate. This activity maps to the 2.7-kilobase-pair WZhet fragment. The BZLF1 open reading frame, present within WZhet as well as in the standard viral BamHI Z fragment, encodes the protein ZEBRA, which induces viral replication. Using gene transfers into Burkitt lymphoma cells, we now demonstrate that rearranged sequences juxtaposed to BZLF1 in het DNA facilitate expression of ZEBRA protein. Two stretches of EBV sequences within a palindromic region of het DNA contain positive regulatory elements. One set, derived from the viral large internal repeat, is newly positioned upstream of BZLF1; the second set is downstream of BZLF1 in het DNA. The capacity of defective HR-1 viruses to disrupt latency of the standard EBV genome is due to abnormal regulation of the BZLF1 gene as a result of genomic rearrangements.

MeSH Terms
Base Sequence Cell Line Chromosome Deletion Deoxyribonuclease EcoRI/metabolism Enhancer Elements, Genetic Gene Expression Regulation Herpesvirus 4, Human/genetics Promoter Regions, Genetic Simian virus 40/genetics Transfection Viral Proteins/genetics Virus Replication
Chemicals
Viral Proteins Deoxyribonuclease EcoRI
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rooney C
Department of Epidemiology, Yale University School of Medicine, New Haven, CT 06510.
Taylor N
Countryman J
Jenson H
Kolman J
Miller G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.24.9801
Published
1988-12-00
Pages
9801-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282869
Subset
IM
Grants
NCI NIH HHS · CA12055 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.24.9801 PMC Full Text View journal details
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