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PMID: 1312615 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Inhibition of poliovirus RNA synthesis by brefeldin A.

Journal of virology ·Vol. 66 ·No. 4 ·1992-04-00 ·Pages 1985-94

Maynell LA, Kirkegaard K, Klymkowsky MW

Abstract

Brefeldin A (BFA), a fungal metabolite that blocks transport of newly synthesized proteins from the endoplasmic reticulum, was found to inhibit poliovirus replication 10(5)- to 10(6)-fold. BFA does not inhibit entry of poliovirus into the cell or translation of viral RNA. Poliovirus RNA synthesis, however, is completely inhibited by BFA. A specific class of membranous vesicles, with which the poliovirus replication complex is physically associated, is known to proliferate in poliovirus-infected cells. BFA may inhibit poliovirus replication by preventing the formation of these vesicles.

MeSH Terms
Adenoviridae/drug effects,genetics,physiology Antiviral Agents/pharmacology Brefeldin A Cyclopentanes/pharmacology Fluorescent Antibody Technique HeLa Cells Humans Kinetics Lipids/biosynthesis Poliovirus/drug effects,genetics,metabolism,physiology Protein Biosynthesis RNA, Viral/biosynthesis Virus Replication/drug effects,genetics
Chemicals
Antiviral Agents Cyclopentanes Lipids RNA, Viral Brefeldin A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Maynell L A
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
Kirkegaard K
Klymkowsky M W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.4.1985-1994.1992
Published
1992-04-00
Pages
1985-94
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288987
Subset
IM
Grants
NIAID NIH HHS · AI-25166 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.4.1985-1994.1992 PMC Full Text View journal details
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