Home LiteratureArticle Details
PMID: 2548189 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Translation of glucose-regulated protein 78/immunoglobulin heavy-chain binding protein mRNA is increased in poliovirus-infected cells at a time when cap-dependent translation of cellular mRNAs is inhibited.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 15 ·1989-08-00 ·Pages 5795-9

Sarnow P

Abstract

All cellular cytoplasmic mRNAs carry a 7-methylguanylate cap attached to their 5' ends. This cap structure is recognized by cap-binding proteins that then direct the binding of ribosomal subunits to this 5'-end complex. Poliovirus, a plus-stranded RNA virus, interferes with this cellular translation process by proteolytically inactivating the cap-binding protein complex. Subsequently the viral mRNA can be translated by an initiation process in which ribosomes bind internally to the mRNA [Pelletier, J. & Sonenberg, N. (1988) Nature (London) 334, 320-325], obviating cap-dependent translation. At least one cellular mRNA, encoding a heat shock-like protein, glucose-regulated protein 78/immunoglobulin heavy-chain binding protein, has been discovered to be translated at an increased rate in poliovirus-infected cells at a time when the translation of other cellular mRNAs is inhibited. The glucose-regulated protein 78/immunoglobulin heavy-chain binding protein mRNA thus exemplifies a cellular mRNA that is translated at a specifically enhanced rate by an as-yet-unresolved cap-independent initiation process in cells when the cap-binding protein complex is not functional.

MeSH Terms
Carrier Proteins/biosynthesis,genetics,isolation & purification Cell Transformation, Viral Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Endoplasmic Reticulum Chaperone BiP HeLa Cells/metabolism Heat-Shock Proteins Humans Molecular Chaperones Molecular Weight Poliovirus/genetics Protein Biosynthesis RNA Caps/genetics RNA, Messenger/genetics
Chemicals
Carrier Proteins Endoplasmic Reticulum Chaperone BiP Heat-Shock Proteins Molecular Chaperones RNA Caps RNA, Messenger
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Sarnow P
Department of Biochemistry, University of Colorado Health Sciences Center, Denver 80262.
References (40)
40 references, click to expand
  1. Capped mRNAs with reduced secondary structure can function in extracts from poliovirus-infected cells.
    Mol Cell Biol. 1982 Dec;2(12):1633-8 PMID: 14582204
  2. Regulation of translation by poliovirus.
    Adv Virus Res. 1987;33:175-204 PMID: 3035905
  3. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  4. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.
    Cell. 1977 Sep;12(1):1-8 PMID: 902310
  5. Spliced segments at the 5' terminus of adenovirus 2 late mRNA.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171-5 PMID: 269380
  6. Infection with paramyxoviruses stimulates synthesis of cellular polypeptides that are also stimulated in cells transformed by Rous sarcoma virus or deprived of glucose.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6120-4 PMID: 216013
  7. In cultured chick embryo fibroblasts the hexose transport components are not the 75 000 and 95 000 dalton polypeptides synthesized following glucose deprivation.
    Can J Biochem. 1980 Oct;58(10):1179-88 PMID: 7459679
  8. Simian virus 40 tandem repeated sequences as an element of the early promoter.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):943-7 PMID: 6262784
  9. Primary structure, gene organization and polypeptide expression of poliovirus RNA.
    Nature. 1981 Jun 18;291(5816):547-53 PMID: 6264310
  10. Highly conserved glucose-regulated protein in hamster and chicken cells: preliminary characterization of its cDNA clone.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4922-5 PMID: 6946438
  11. Cloned poliovirus complementary DNA is infectious in mammalian cells.
    Science. 1981 Nov 20;214(4523):916-9 PMID: 6272391
  12. Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells.
    Cell. 1982 Feb;28(2):387-94 PMID: 6277513
  13. Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage at glutamine-glycine pairs.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):3973-7 PMID: 6287457
  14. Newcastle disease virus stimulates the cellular accumulation of stress (heat shock) mRNAs and proteins.
    J Virol. 1982 Nov;44(2):703-7 PMID: 7143579
  15. Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.
    J Biol Chem. 1982 Dec 25;257(24):14806-10 PMID: 6294080
  16. Transcriptional regulation of two genes specifically induced by glucose starvation in a hamster mutant fibroblast cell line.
    J Biol Chem. 1983 Jan 10;258(1):597-603 PMID: 6294115
  17. Identification and characterization of an immunologically conserved adenovirus early region 11,000 Mr protein and its association with the nuclear matrix.
    J Mol Biol. 1982 Dec 15;162(3):565-83 PMID: 7166756
  18. Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.
    Nucleic Acids Res. 1984 Jun 25;12(12):5123-41 PMID: 6330698
  19. Synthesis of heat-shock proteins in HeLa cells: inhibition by virus infection.
    Virology. 1984 Aug;137(1):150-9 PMID: 6089412
  20. Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 alpha subunit subsequent to infection.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4321-5 PMID: 2989814
  21. A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):571-5 PMID: 3003739
  22. A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1777-81 PMID: 3006071
  23. Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas.
    J Cell Biol. 1986 May;102(5):1558-66 PMID: 3084497
  24. A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein.
    Cell. 1986 Jun 6;45(5):761-70 PMID: 3011278
  25. The presence of malfolded proteins in the endoplasmic reticulum signals the induction of glucose-regulated proteins.
    Nature. 1988 Mar 31;332(6163):462-4 PMID: 3352747
  26. The adenovirus tripartite leader may eliminate the requirement for cap-binding protein complex during translation initiation.
    J Virol. 1988 Jun;62(6):2059-66 PMID: 2835510
  27. An RNA sequence of hundreds of nucleotides at the 5' end of poliovirus RNA is involved in allowing viral protein synthesis.
    J Virol. 1988 Jul;62(7):2291-9 PMID: 2836612
  28. A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation.
    J Virol. 1988 Aug;62(8):2636-43 PMID: 2839690
  29. Poliovirus mutant that contains a cold-sensitive defect in viral RNA synthesis.
    J Virol. 1988 Aug;62(8):2922-8 PMID: 2839711
  30. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA.
    Nature. 1988 Jul 28;334(6180):320-5 PMID: 2839775
  31. Human gene encoding the 78,000-dalton glucose-regulated protein and its pseudogene: structure, conservation, and regulation.
    DNA. 1988 May;7(4):275-86 PMID: 2840249
  32. 78-kilodalton glucose-regulated protein is induced in Rous sarcoma virus-transformed cells independently of glucose deprivation.
    Mol Cell Biol. 1988 Jul;8(7):2675-80 PMID: 2841586
  33. ELECTRON MICROSCOPIC STUDY OF THE FORMATION OF POLIOVIRUS.
    Virology. 1965 Jul;26:379-89 PMID: 14319710
  34. An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.
    Cell. 1986 Jul 18;46(2):291-300 PMID: 3087629
  35. Poliovirus mutant that does not selectively inhibit host cell protein synthesis.
    Mol Cell Biol. 1985 Nov;5(11):2913-23 PMID: 3018486
  36. Speculations on the functions of the major heat shock and glucose-regulated proteins.
    Cell. 1986 Sep 26;46(7):959-61 PMID: 2944601
  37. Genetic complementation among poliovirus mutants derived from an infectious cDNA clone.
    J Virol. 1986 Dec;60(3):1040-9 PMID: 3023656
  38. Poliovirus temperature-sensitive mutant containing a single nucleotide deletion in the 5'-noncoding region of the viral RNA.
    Virology. 1986 Dec;155(2):498-507 PMID: 3024397
  39. Proteolysis of the p220 component of the cap-binding protein complex is not sufficient for complete inhibition of host cell protein synthesis after poliovirus infection.
    J Virol. 1987 Apr;61(4):986-91 PMID: 3029432
  40. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.15.5795
Published
1989-08-00
Pages
5795-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297717
Subset
IM
Grants
NIA NIH HHS · AG-07347 · United States
NIAID NIH HHS · AI-25105 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.15.5795 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2