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PMID: 2989814 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 alpha subunit subsequent to infection.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 13 ·1985-07-00 ·Pages 4321-5

Schneider RJ, Safer B, Munemitsu SM, Samuel CE, Shenk T

Abstract

The virus-associated VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of mRNAs late after infection. Deletion mutant dl331 fails to produce this RNA and, as a result, grows poorly. Three lines of evidence suggest that VAI RNA facilitates translation by preventing inactivation of the function of eukaryotic initiation factor 2 (eIF-2). First, the mutant's translational defect can be relieved by addition of eIF-2 or eIF-2B (GTP recycling factor). Second, extracts of mutant-infected cells exhibit enhanced protein P1/eIF-2 alpha subunit kinase activity. Third, dl331 can grow with nearly normal kinetics in cells that do not express the kinase.

MeSH Terms
Adenoviridae Infections/microbiology Adenovirus Infections, Human/microbiology Adenoviruses, Human/genetics,growth & development Cell Line Electrophoresis, Polyacrylamide Gel Eukaryotic Initiation Factor-2 Female Humans Kinetics Mutation Peptide Initiation Factors/metabolism Phosphorylation Pregnancy Protein Biosynthesis Protein Kinases/metabolism Proteins/metabolism RNA, Viral/metabolism
Chemicals
Eukaryotic Initiation Factor-2 Peptide Initiation Factors Proteins RNA, Viral Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schneider R J
Safer B
Munemitsu S M
Samuel C E
Shenk T
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.13.4321
Published
1985-07-00
Pages
4321-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390405
Subset
IM
Grants
NIAID NIH HHS · AI 12520 · United States
NIAID NIH HHS · AI 20611 · United States
NCI NIH HHS · CA 07227 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.13.4321 PMC Full Text View journal details
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