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PMID: 6930642 Published · ppublish English Journal Article

Structure of genes for virus-associated RNAI and RNAII of adenovirus type 2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 2424-8

Akusjärvi G, Mathews MB, Andersson P, Vennström B, Pettersson U

Abstract

A DNA sequence, 552 base pairs in length, encoding the two "virus-associated" (VA) RNAs of adenovirus type 2 is presented. Comparison of the oligonucleotide maps of VA RNAI and VA RNII with the established sequence permits identification of the genes for these RNAs. VA RNAI is 157-160 nucleotides long and VA RNAII 158-163 nucleotides long, depending on the exact length of their heterogeneous 3' end. The genes are separated by a spacer of about 98 nucleotides. The RNAs exhibit scattered regions of primary sequence homology and can adopt secondary structures which resemble each other closely in their configuration and stability. VA RNAII is also capable of assuming a different configuration that is energetically more favorable. The data suggest that the two RNA genes may have arisen by duplication of an ancestral gene and that the folding of the RNA chain may be of importance for the function of VA RNAs. Hypothetical RNA polymerase III recognition sequences and the coding potential of the region are discussed.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Biological Evolution Genes Genes, Viral Hydrogen Bonding Nucleic Acid Conformation Operon RNA Polymerase III/metabolism RNA, Viral/genetics
Chemicals
RNA, Viral RNA Polymerase III
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Akusjärvi G
Mathews M B
Andersson P
Vennström B
Pettersson U
References (33)
33 references, click to expand
  1. Nucleic acid homology studies of adenovirus type 7-SV40 interactions.
    Proc Natl Acad Sci U S A. 1966 Feb;55(2):336-41 PMID: 4287386
  2. The nucleotide sequence of a low molecular weight ribonucleic acid from cells infected with adenovirus 2.
    J Biol Chem. 1971 Nov 25;246(22):6991-7009 PMID: 4331202
  3. Transcription of the adenovirus genome by an -amanitine-sensitive ribonucleic acid polymerase in HeLa cells.
    J Virol. 1972 Apr;9(4):621-6 PMID: 5021308
  4. State of adenovirus 2 deoxyribonucleic acid in the nucleus and its mode of transcription: studies with isolated viral deoxyribonucleic acid-protein complexes and isolated nuclei.
    J Virol. 1972 Apr;9(4):627-35 PMID: 5021309
  5. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  6. Location of sequences on the adenovirus genome coding for the 5.5S RNA.
    Cell. 1975 Sep;6(1):1-4 PMID: 1164730
  7. Genes for VA-RNA in adenovirus 2.
    Cell. 1975 Oct;6(2):223-9 PMID: 1182803
  8. Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure.
    Anal Biochem. 1976 May 7;72:433-46 PMID: 942063
  9. Low molecular weight viral RNAs transcribed by RNA polymerase III during adenovirus 2 infection.
    Cell. 1976 Apr;7(4):557-66 PMID: 954087
  10. A new species of virus-coded low molecular weight RNA from cells infected with adenovirus type 2.
    Cell. 1976 Apr;7(4):585-93 PMID: 954088
  11. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  12. Studies of low molecular weight RNA from cells infected with adenovirus 2. I. The sequences at the 3' end of VA-RNA I.
    J Biol Chem. 1977 Dec 25;252(24):9032-42 PMID: 318160
  13. Studies of low molecular weight RNA from cells infected with adenovirus 2. II. Heterogeneity at the 5' end of VA-RNA I.
    J Biol Chem. 1977 Dec 25;252(24):9043-6 PMID: 925035
  14. Studies of low molecular weight RNA from cells infected with adenovirus 2. III. The sequence of the promoter for VA-RNA I.
    J Biol Chem. 1977 Dec 25;252(24):9047-54 PMID: 200627
  15. The low molecular weight of RNAs of adenovirus 2-infected cells.
    J Mol Biol. 1978 Feb 25;119(2):293-328 PMID: 633371
  16. Two initiation sites for adenovirus 5.5S RNA.
    Nucleic Acids Res. 1978 Jan;5(1):195-204 PMID: 643608
  17. Initiation of transcription in nuclei isolated from adenovirus infected cells.
    Nucleic Acids Res. 1978 Jan;5(1):205-19 PMID: 643609
  18. The spliced structures of adenovirus 2 fiber message and the other late mRNAs.
    Cell. 1978 Oct;15(2):497-510 PMID: 719751
  19. How do eucaryotic ribosomes select initiation regions in messenger RNA?
    Cell. 1978 Dec;15(4):1109-23 PMID: 215319
  20. Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.
    Cell. 1978 Dec;15(4):1145-56 PMID: 264240
  21. Coincidence of the promoter and capped 5' terminus of RNA from the adenovirus 2 major late transcription unit.
    Cell. 1978 Dec;15(4):1463-75 PMID: 729003
  22. Steps in the processing of Ad2 mRNA: poly(A)+ nuclear sequences are conserved and poly(A) addition precedes splicing.
    Cell. 1978 Dec;15(4):1477-93 PMID: 729004
  23. Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
    J Biol Chem. 1979 Jul 10;254(13):6163-73 PMID: 447704
  24. Sequence analysis of adenovirus DNA: complete nucleotide sequence of the spliced 5' noncoding region of adenovirus 2 hexon messenger RNA.
    Cell. 1979 Apr;16(4):841-50 PMID: 455452
  25. Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA.
    Cell. 1979 Apr;16(4):851-61 PMID: 455453
  26. Delimitation of a promoter for RNA polymerase III by means of a functional test.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2590-4 PMID: 288049
  27. BKV splice sequences based on analysis of preferred donor and acceptor sites.
    Nucleic Acids Res. 1979 Jul 25;6(10):3387-98 PMID: 225729
  28. A mutation which alters initiation of transcription by RNA polymerase III on the Ad5 chromosome.
    Cell. 1979 Dec;18(4):947-54 PMID: 519773
  29. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
    Cell. 1980 Jan;19(1):13-25 PMID: 7357599
  30. A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
    Cell. 1980 Jan;19(1):27-35 PMID: 7357604
  31. Determination and analysis of adenovirus-2 DNA sequences which may include signals for late messenger RNA processing.
    J Mol Biol. 1979 Dec 5;135(2):413-33 PMID: 318167
  32. A tRNA gene of Xenopus laevis contains at least two sites promoting transcription.
    Nucleic Acids Res. 1979 Dec 11;7(7):1749-63 PMID: 537910
  33. The gene for polypeptide IX of adenovirus type 2 and its unspliced messenger RNA.
    Cell. 1980 Mar;19(3):671-81 PMID: 6985473
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.5.2424
Published
1980-05-00
Pages
2424-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349411
Subset
IM
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1073/pnas.77.5.2424 PMC Full Text View journal details
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