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PMID: 288049 Published · ppublish English Journal Article

Delimitation of a promoter for RNA polymerase III by means of a functional test.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 6 ·1979-06-00 ·Pages 2590-4

Telford JL, Kressmann A, Koski RA, Grosschedl R, Müller F, Clarkson SG, Birnstiel ML

Abstract

A Xenopus laevis DNA segment containing the structural gene for tRNA1Met and 22 base pairs at the 5' side of the gene is active in tRNA production. The DNA segment was ligated to sea urchin histone DNA and was also inserted into plasmid pCR1. Both recombinant DNAs were shown to produce mature tRNA1Met at a high rate when injected into centrifuged Xenopus oocytes.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes DNA, Recombinant/metabolism DNA-Directed RNA Polymerases/biosynthesis Female Methionine Oocytes/metabolism RNA Polymerase III/biosynthesis RNA, Transfer/biosynthesis Transcription, Genetic Xenopus
Chemicals
DNA, Recombinant RNA, Transfer Methionine DNA-Directed RNA Polymerases RNA Polymerase III DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Telford J L
Kressmann A
Koski R A
Grosschedl R
Müller F
Clarkson S G
Birnstiel M L
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.6.2590
Published
1979-06-00
Pages
2590-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383653
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.6.2590 PMC Full Text View journal details
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