Home LiteratureArticle Details
PMID: 2835510 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The adenovirus tripartite leader may eliminate the requirement for cap-binding protein complex during translation initiation.

Journal of virology ·Vol. 62 ·No. 6 ·1988-06-00 ·Pages 2059-66

Dolph PJ, Racaniello V, Villamarin A, Palladino F, Schneider RJ

Abstract

The adenovirus tripartite leader is a 200-nucleotide 5' noncoding region that is found on all late viral mRNAs. This segment is required for preferential translation of viral mRNAs at late times during infection. Most tripartite leader-containing mRNAs appear to exhibit little if any requirement for intact cap-binding protein complex, a property previously established only for uncapped poliovirus mRNAs and capped mRNAs with minimal secondary structure. The tripartite leader also permits the translation of mRNAs in poliovirus-infected cells in the apparent absence of active cap-binding protein complex and does not require any adenovirus gene products for this activity. The preferential translation of viral late mRNAs may involve this unusual property.

MeSH Terms
Adenoviruses, Human/genetics Animals Carrier Proteins/physiology Cell Line Humans Macromolecular Substances Nucleic Acid Conformation Poliovirus/genetics Protein Biosynthesis RNA Cap-Binding Proteins RNA, Messenger/genetics RNA, Viral/genetics Time Factors Viral Proteins/genetics
Chemicals
Carrier Proteins Macromolecular Substances RNA Cap-Binding Proteins RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dolph P J
Department of Biochemistry, New York University Medical Center, New York 10016.
Racaniello V
Villamarin A
Palladino F
Schneider R J
References (54)
54 references, click to expand
  1. Capped mRNAs with reduced secondary structure can function in extracts from poliovirus-infected cells.
    Mol Cell Biol. 1982 Dec;2(12):1633-8 PMID: 14582204
  2. Inhibition of transcription factor activity by poliovirus.
    Cell. 1981 Dec;27(3 Pt 2):555-61 PMID: 6086108
  3. Adenovirus polypeptide synthesis in the presence of non-replicating poliovirus.
    J Gen Virol. 1974 Aug;24(2):261-79 PMID: 4368375
  4. Cellular protein synthesis shutoff by mengovirus: translation of nonviral and viral mRNA's in extracts from uninfected and infected Ehrlich ascites tumor cells.
    J Virol. 1976 Apr;18(1):182-94 PMID: 176460
  5. Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
    J Gen Virol. 1977 Jul;36(1):59-74 PMID: 886304
  6. Spliced segments at the 5' terminus of adenovirus 2 late mRNA.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171-5 PMID: 269380
  7. Adenovirus-2 messengers--an example of baroque molecular architecture.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:531-53 PMID: 277362
  8. Comparison of initiation rates of encephalomyocarditis virus and host protein synthesis in infected cells.
    J Virol. 1978 Sep;27(3):640-7 PMID: 212586
  9. Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells.
    Cell. 1979 Jul;17(3):683-9 PMID: 476833
  10. Inhibition of HeLa cell protein synthesis during adenovirus infection. Restriction of cellular messenger RNA sequences to the nucleus.
    J Mol Biol. 1979 Jun 25;131(2):353-73 PMID: 226719
  11. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  12. Influence of mRNA secondary structure on binding and migration of 40S ribosomal subunits.
    Cell. 1980 Jan;19(1):79-90 PMID: 7357609
  13. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  14. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  15. SV40-transformed simian cells support the replication of early SV40 mutants.
    Cell. 1981 Jan;23(1):175-82 PMID: 6260373
  16. Gene and mRNA for precursor polypeptide VI from adenovirus type 2.
    J Virol. 1981 May;38(2):469-82 PMID: 6985479
  17. The role of mRNA competition in regulating translation. I. Demonstration of competition in vivo.
    J Biol Chem. 1981 Nov 25;256(22):11739-46 PMID: 7298628
  18. Control of messenger RNA concentration by differential cytoplasmic half-life. Adenovirus messenger RNAs from transcription units 1A and 1B.
    J Mol Biol. 1981 May 25;148(3):231-51 PMID: 7299816
  19. Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells.
    Cell. 1982 Feb;28(2):387-94 PMID: 6277513
  20. Inactivation of cap-binding proteins accompanies the shut-off of host protein synthesis by poliovirus.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3447-51 PMID: 6954488
  21. Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.
    J Biol Chem. 1982 Dec 25;257(24):14806-10 PMID: 6294080
  22. Role of mRNA competition in regulating translation: further characterization of mRNA discriminatory initiation factors.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):663-7 PMID: 6572361
  23. New initiation factor activity required for globin mRNA translation.
    J Biol Chem. 1983 May 10;258(9):5804-10 PMID: 6853548
  24. Control of cellular gene expression during adenovirus infection: induction and shut-off of dihydrofolate reductase gene expression by adenovirus type 2.
    Mol Cell Biol. 1983 May;3(5):819-28 PMID: 6865943
  25. Involvement of eukaryotic initiation factor 4A in the cap recognition process.
    J Biol Chem. 1983 Sep 25;258(18):11398-403 PMID: 6604056
  26. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  27. 5'-Conformation of capped alfalfa mosaic virus ribonucleic acid 4 may reflect its independence of the cap structure or of cap-binding protein for efficient translation.
    Biochemistry. 1983 Oct 25;22(22):5157-64 PMID: 6317016
  28. Adenovirus type 5 early region 1b gene product is required for efficient shutoff of host protein synthesis.
    J Virol. 1984 Apr;50(1):202-12 PMID: 6142122
  29. Intracellular transport and secretion of hepatitis B surface antigen in mammalian cells.
    J Virol. 1984 Aug;51(2):346-53 PMID: 6748160
  30. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.
    Nucleic Acids Res. 1984 Jul 25;12(14):5707-17 PMID: 6589587
  31. Demonstration in vitro that eucaryotic initiation factor 3 is active but that a cap-binding protein complex is inactive in poliovirus-infected HeLa cells.
    J Virol. 1984 Sep;51(3):832-7 PMID: 6088805
  32. Extreme instability of myc mRNA in normal and transformed human cells.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7046-50 PMID: 6594679
  33. Insertion mutagenesis to increase secondary structure within the 5' noncoding region of a eukaryotic mRNA reduces translational efficiency.
    Cell. 1985 Mar;40(3):515-26 PMID: 2982496
  34. Identification of the components necessary for adenovirus translational control and their utilization in cDNA expression vectors.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):689-93 PMID: 2983309
  35. Translational control by adenovirus: lack of virus-associated RNAI during adenovirus infection results in phosphorylation of initiation factor eIF-2 and inhibition of protein synthesis.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):1959-63 PMID: 3856874
  36. The host protein required for in vitro replication of poliovirus is a protein kinase that phosphorylates eukaryotic initiation factor-2.
    Cell. 1985 Apr;40(4):913-21 PMID: 2985268
  37. Isolation and structural characterization of cap-binding proteins from poliovirus-infected HeLa cells.
    J Virol. 1985 May;54(2):515-24 PMID: 2985814
  38. ATP-dependent unwinding of messenger RNA structure by eukaryotic initiation factors.
    J Biol Chem. 1985 Jun 25;260(12):7651-8 PMID: 3838990
  39. Effect of the tripartite leader on synthesis of a non-viral protein in an adenovirus 5 recombinant.
    Nucleic Acids Res. 1985 Feb 11;13(3):841-57 PMID: 3839074
  40. Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 alpha subunit subsequent to infection.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4321-5 PMID: 2989814
  41. Adenovirus early region 4 encodes functions required for efficient DNA replication, late gene expression, and host cell shutoff.
    J Virol. 1985 Oct;56(1):250-7 PMID: 4032537
  42. Selective translation of heat shock mRNA in Drosophila melanogaster depends on sequence information in the leader.
    EMBO J. 1985 Aug;4(8):2053-60 PMID: 2933251
  43. Purification of a terminal uridylyltransferase that acts as host factor in the in vitro poliovirus replicase reaction.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):221-5 PMID: 2417240
  44. Translational and transcriptional control elements in the untranslated leader of the heat-shock gene hsp22.
    Cell. 1986 Feb 14;44(3):429-38 PMID: 3943132
  45. Influences of mRNA secondary structure on initiation by eukaryotic ribosomes.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2850-4 PMID: 3458245
  46. Poliovirus mutant that does not selectively inhibit host cell protein synthesis.
    Mol Cell Biol. 1985 Nov;5(11):2913-23 PMID: 3018486
  47. Photochemical cross-linking of cap binding proteins to eucaryotic mRNAs: effect of mRNA 5' secondary structure.
    Mol Cell Biol. 1985 Nov;5(11):3222-30 PMID: 3837842
  48. Influence of 5' proximal secondary structure on the translational efficiency of eukaryotic mRNAs and on their interaction with initiation factors.
    J Biol Chem. 1986 Oct 25;261(30):13979-89 PMID: 3771516
  49. The adenovirus E1B-55K transforming polypeptide modulates transport or cytoplasmic stabilization of viral and host cell mRNAs.
    Mol Cell Biol. 1986 Feb;6(2):470-6 PMID: 2946932
  50. Proteolysis of the p220 component of the cap-binding protein complex is not sufficient for complete inhibition of host cell protein synthesis after poliovirus infection.
    J Virol. 1987 Apr;61(4):986-91 PMID: 3029432
  51. Adenovirus late protein synthesis is resistant to the inhibition of translation induced by poliovirus.
    J Biol Chem. 1987 May 25;262(15):7328-34 PMID: 3034890
  52. Regulation of translation by poliovirus.
    Adv Virus Res. 1987;33:175-204 PMID: 3035905
  53. Impact of virus infection on host cell protein synthesis.
    Annu Rev Biochem. 1987;56:317-32 PMID: 3113326
  54. Regulation of protein synthesis in HeLa cells. 3. Inhibition during poliovirus infection.
    J Virol. 1971 Nov;8(5):661-8 PMID: 4332137
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.6.2059-2066.1988
Published
1988-06-00
Pages
2059-66
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253291
Subset
IM
Grants
NIAID NIH HHS · AI 20017 · United States
NCI NIH HHS · CA-42357 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.6.2059-2066.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2