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PMID: 1313045 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis.

Journal of clinical microbiology ·Vol. 30 ·No. 3 ·1992-03-00 ·Pages 577-80

Goering RV, Winters MA

Abstract

We report a rapid method for the isolation of intact chromosomal DNA from gram-positive cocci that is suitable for in situ restriction endonuclease digestion in agarose blocks. When combined with a rapid field inversion gel electrophoresis protocol, this approach allows the preparation and electrophoretic analysis of chromosomal restriction fragments produced by rare-cutting enzymes in a total time period of 2 days from start to finish. The utility of the method is demonstrated in the epidemiological evaluation of Staphylococcus epidermidis clusters from two hospitals as well as of additional representative staphylococci and enterococci.

MeSH Terms
DNA Restriction Enzymes DNA, Bacterial/isolation & purification Disease Outbreaks Electrophoresis, Agar Gel/methods Epidemiologic Methods Evaluation Studies as Topic Gram-Positive Cocci/isolation & purification Humans Staphylococcal Infections/epidemiology,microbiology Staphylococcus epidermidis/isolation & purification
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goering R V
Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178.
Winters M A
References (16)
16 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.3.577-580.1992
Published
1992-03-00
Pages
577-80
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265112
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.3.577-580.1992 PMC Full Text View journal details
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