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PMID: 1691206 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Rapid field inversion gel electrophoresis in combination with an rRNA gene probe in the epidemiological evaluation of staphylococci.

Journal of clinical microbiology ·Vol. 28 ·No. 3 ·1990-03-00 ·Pages 426-9

Goering RV, Duensing TD

Abstract

A rapid field inversion gel electrophoresis (FIGE) protocol was combined with an rRNA gene probe in the analysis of staphylococci that were difficult to study epidemiologically by conventional means. The following groups of clinical isolates were examined: (i) predominantly antibiotic-susceptible Staphylococcus aureus strains containing no detectable plasmids and unresponsive to bacteriophage typing and (ii) methicillin-resistant Staphylococcus epidermidis strains carrying a single plasmid ca. 30 kilobases in size. The results indicated that strain interrelationships could be established on the basis of SmaI-generated chromosomal restriction fragment length polymorphisms (RFLPs) analyzed by FIGE. RFLP analysis of strains known to be unrelated established the importance of minor differences in DNA banding patterns as indicators of strain dissimilarities. Hybridization studies with an rRNA gene probe confirmed this conclusion. These results suggest that FIGE analysis of chromosomal RFLPs (especially in combination with molecular probes) is an important addition to the armamentarium of molecular epidemiology.

MeSH Terms
DNA, Bacterial/analysis Electrophoresis, Agar Gel Humans Nucleic Acid Hybridization Plasmids Polymorphism, Restriction Fragment Length RNA Probes RNA, Bacterial/genetics RNA, Ribosomal/genetics Staphylococcal Infections/epidemiology Staphylococcus aureus/classification,genetics Staphylococcus epidermidis/classification,genetics
Chemicals
DNA, Bacterial RNA Probes RNA, Bacterial RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goering R V
Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178.
Duensing T D
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.28.3.426-429.1990
Published
1990-03-00
Pages
426-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC269636
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/jcm.28.3.426-429.1990 PMC Full Text View journal details
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