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PMID: 1316607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immortal rat hippocampal cell lines exhibit neuronal and glial lineages and neurotrophin gene expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 10 ·1992-05-15 ·Pages 4373-7

Eves EM, Tucker MS, Roback JD, Downen M, Rosner MR, Wainer BH

Abstract

Clonal cell lines of rat embryonic hippocampal origin have been developed by using retroviral transduction of temperature-sensitive simian virus 40 large tumor antigens. The cell lines undergo morphological differentiation at the nonpermissive temperature and in response to differentiating agents. Immunocytochemical analysis indicates that various lines are derived from progenitors of neuronal, glial, and bipotential lineages. Selected neuronal lines differentiate in response to diffusible factors released by primary glia, and one line of glial lineage supports the maturation of primary neurons in culture. Selected cell lines exhibit different patterns of neurotrophin gene expression that change after differentiation. In some lines, the relative levels of neurotrophin 3 and brain-derived neurotrophic factor message expression may reflect the developmental or regional differential expression seen for these genes in the hippocampus in situ. These hippocampal cell lines, which express markers indicative of commitment to neuronal or glial lineages, are valuable for studies of development and plasticity in these lineages, as well as for studies of the regulation of neural trophic interactions.

MeSH Terms
3T3 Cells Animals Antigens, Polyomavirus Transforming/genetics Brain-Derived Neurotrophic Factor Cell Line, Transformed Cell Transformation, Viral Cells, Cultured Clone Cells DNA Replication Embryo, Mammalian Gene Expression Hippocampus/physiology Mice Nerve Growth Factors/genetics Nerve Tissue Proteins/genetics Neuroglia/physiology Neurons/physiology RNA, Messenger/genetics,metabolism Rats Simian virus 40/genetics Thymidine/metabolism
Chemicals
Antigens, Polyomavirus Transforming Brain-Derived Neurotrophic Factor Nerve Growth Factors Nerve Tissue Proteins RNA, Messenger Thymidine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Eves E M
Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637.
Tucker M S
Roback J D
Downen M
Rosner M R
Wainer B H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.10.4373
Published
1992-05-15
Pages
4373-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49084
Subset
IM
Grants
NIGMS NIH HHS · GM07151 · United States
NINDS NIH HHS · NS25787 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.10.4373 PMC Full Text View journal details
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