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PMID: 1318413 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human cytomegalovirus contains a tegument protein that enhances transcription from promoters with upstream ATF and AP-1 cis-acting elements.

Journal of virology ·Vol. 66 ·No. 7 ·1992-07-00 ·Pages 4434-44

Liu B, Stinski MF

Abstract

The tegument proteins of human cytomegalovirus are introduced into cells as components of infectious virus. The tegument proteins may affect viral and cellular transcription prior to the synthesis of the immediate-early viral regulatory proteins. The phosphorylated tegument protein of 71 kDa (pp71) is reported to be encoded by the UL82 gene. The UL82 gene products transactivated promoters containing upstream ATF or AP-1 binding sites. In contrast, the phosphorylated tegument protein of 65 kDa (pp65), encoded by the UL83 gene, had no detectable effect on these promoters. Enhancement by UL82 of downstream transcription was directly proportional to the number of upstream ATF sites. Response to UL82 transactivation was abolished by mutation of the ATF site. Mutation in the carboxy-terminal region of UL82 also eliminated transactivation. Even though the major immediate-early promoter of human cytomegalovirus is a strong enhancer-containing promoter, UL82 further enhanced its transcription as much as 20-fold. The mechanism of UL82 enhancement of transcription from viral or cellular promoters is not known, but the enhancement may be mediated by triggering one of the protein kinase signaling pathways, increasing the affinity of ATF or AP-1 for the target sequence, or stabilizing the complex between the eucaryotic transcription factor and the target sequence.

MeSH Terms
Activating Transcription Factors Amino Acid Sequence Antigens, Viral/genetics Base Sequence Blood Proteins/metabolism Cytomegalovirus/genetics,metabolism DNA, Viral Humans Immediate-Early Proteins Molecular Sequence Data Phosphoproteins/metabolism Promoter Regions, Genetic Proto-Oncogene Proteins c-jun/metabolism Regulatory Sequences, Nucleic Acid Restriction Mapping Transcription Factors/metabolism Transcription, Genetic Transcriptional Activation Viral Envelope Proteins Viral Matrix Proteins/metabolism Viral Proteins/genetics,metabolism
Chemicals
Activating Transcription Factors Antigens, Viral Blood Proteins DNA, Viral Immediate-Early Proteins Phosphoproteins Proto-Oncogene Proteins c-jun Transcription Factors Viral Envelope Proteins Viral Matrix Proteins Viral Proteins cytomegalovirus matrix protein 65kDa immediate-early proteins, cytomegalovirus cytomegalovirus phosphoprotein 71kDa
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Liu B
Department of Microbiology, College of Medicine, University of Iowa, Iowa City 52242.
Stinski M F
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.7.4434-4444.1992
Published
1992-07-00
Pages
4434-44
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241251
Subset
IM
Grants
NIAID NIH HHS · AI 13562 · United States
NICHD NIH HHS · HD 19937 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.7.4434-4444.1992 PMC Full Text View journal details
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