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PMID: 1323850 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Skin test to assess virus-specific cytotoxic T-cell activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 16 ·1992-08-15 ·Pages 7757-61

Kündig TM, Althage A, Hengartner H, Zinkernagel RM

Abstract

A way to assess specific CD8+ T-cell activity in a skin test analogous to the conventional delayed-type hypersensitivity (DTH) reaction for CD4+ T cells is presented. Local injection of viral class I binding peptides caused a specific CD8+ T-cell-mediated DTH in footpads of virally infected mice. The DTH was inducible only during the acute phase of the infection. Apparently because of the short half-life of locally available peptide, only activated CD8+ effector T cells could mediate the reaction. This skin test may prove to be particularly interesting for use in humans to evaluate the activation status of CD8+ T cells during acute viral infections and of memory CD8+ T cells, for example, in chronically active immunopathological disease or infection.

MeSH Terms
Animals CD4 Antigens/immunology CD8 Antigens/immunology Cell Line Hypersensitivity, Delayed Immunity, Cellular Mice Mice, Inbred BALB C Mice, Inbred C57BL Skin Tests T-Lymphocyte Subsets/immunology T-Lymphocytes, Cytotoxic/immunology Time Factors Vesicular stomatitis Indiana virus/immunology
Chemicals
CD4 Antigens CD8 Antigens
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kündig T M
Laboratory of Experimental Immunology, University of Zurich, Switzerland.
Althage A
Hengartner H
Zinkernagel R M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.16.7757
Published
1992-08-15
Pages
7757-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49790
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.16.7757 PMC Full Text View journal details
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