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PMID: 1326945 Published · ppublish English Journal Article

Functional difference between SERCA2a and SERCA2b Ca2+ pumps and their modulation by phospholamban.

The Biochemical journal ·Vol. 286 ( Pt 2) ·1992-09-01 ·Pages 591-5

Verboomen H, Wuytack F, De Smedt H, Himpens B, Casteels R

Abstract

COS 1 cells were transfected with full-length pig stomach sarcoplasmic/endoplasmic reticulum Ca2+ pump (SERCA)2a or SERCA2b cDNA. Ca2+ uptake by microsomes from transfected cells revealed that the Ca2+ affinity of the SERCA2b Ca2+ pump (K0.5 0.17 +/- 0.01 microM) was higher than that of the SERCA2a Ca2+ pump (K0.5 0.31 +/- 0.02 microM). Thapsigargin-sensitivity was found to be identical for the two isoforms. The Ca2+ affinity of both the SERCA2a and SERCA2b Ca2+ pumps was decreased by a factor of two when they were co-expressed with phospholamban.

Related Genes
SERCA2
MeSH Terms
Animals Biological Transport Blotting, Western Calcium/metabolism Calcium-Binding Proteins/pharmacology Calcium-Transporting ATPases/drug effects,genetics,metabolism,physiology Cell Line DNA Electrophoresis, Polyacrylamide Gel Endoplasmic Reticulum/metabolism Gastric Mucosa/metabolism Immunohistochemistry Microsomes/metabolism Sarcoplasmic Reticulum/metabolism Swine Terpenes/pharmacology Thapsigargin Transfection
Chemicals
Calcium-Binding Proteins Terpenes phospholamban Thapsigargin DNA Calcium-Transporting ATPases Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Verboomen H
Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Belgium.
Wuytack F
De Smedt H
Himpens B
Casteels R
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2860591
Published
1992-09-01
Pages
591-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132938
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2860591 PMC Full Text View journal details
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