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PMID: 1328682 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The UL16 gene of human cytomegalovirus encodes a glycoprotein that is dispensable for growth in vitro.

Journal of virology ·Vol. 66 ·No. 11 ·1992-11-00 ·Pages 6609-15

Kaye J, Browne H, Stoffel M, Minson T

Abstract

The UL16 gene of human cytomegalovirus (HCMV) encodes a predicted translation product with features characteristic of glycoproteins (signal and anchor sequences and eight potential N-linked glycosylation sites). Antisera were raised against the UL16 gene product expressed in Escherichia coli as a beta-galactosidase fusion protein. The antisera detected a 50-kDa glycoprotein in HCMV-infected cells that was absent from purified virions. The UL16 glycoprotein was synthesized at early times after infection and accumulated to the highest levels at late times after infection. A recombinant HCMV in which UL16 coding sequences were interrupted by a lacZ expression cassette was constructed by insertional mutagenesis. Analysis of the phenotype of the recombinant virus indicated that the UL16 gene product is nonessential for virus infectivity and growth in tissue culture.

Related Genes
UL16
MeSH Terms
Antibodies, Viral Cell Communication Cells, Cultured Culture Techniques Cytomegalovirus/genetics,growth & development,pathogenicity Cytomegalovirus Infections/genetics DNA, Viral/analysis Fibroblasts/microbiology Genes, Viral/genetics Humans Membrane Glycoproteins/genetics,immunology Membrane Proteins/genetics Mutagenesis, Insertional Phenotype RNA, Viral/analysis Viral Matrix Proteins/genetics,immunology Virion/chemistry Virulence Virus Replication
Chemicals
Antibodies, Viral DNA, Viral Membrane Glycoproteins Membrane Proteins RNA, Viral Viral Matrix Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kaye J
Department of Pathology, University of Cambridge, United Kingdom.
Browne H
Stoffel M
Minson T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.11.6609-6615.1992
Published
1992-11-00
Pages
6609-15
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240156
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.11.6609-6615.1992 PMC Full Text View journal details
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