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PMID: 2831402 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification and expression of a human cytomegalovirus glycoprotein with homology to the Epstein-Barr virus BXLF2 product, varicella-zoster virus gpIII, and herpes simplex virus type 1 glycoprotein H.

Journal of virology ·Vol. 62 ·No. 4 ·1988-04-00 ·Pages 1416-22

Cranage MP, Smith GL, Bell SE, Hart H, Brown C, Bankier AT, Tomlinson P, Barrell BG, Minson TC

Abstract

An open reading frame with the characteristics of a glycoprotein-coding sequence was identified by nucleotide sequencing of human cytomegalovirus (HCMV) genomic DNA. The predicted amino acid sequence was homologous with glycoprotein H of herpes simplex virus type 1 and the homologous protein of Epstein-Barr virus (BXLF2 gene product) and varicella-zoster virus (gpIII). Recombinant vaccinia viruses that expressed this gene were constructed. A glycoprotein of approximately 86 kilodaltons was immunoprecipitated from cells infected with the recombinant viruses and from HCMV-infected cells with a monoclonal antibody that efficiently neutralized HCMV infectivity. In HCMV-infected MRC5 cells, this glycoprotein was present on nuclear and cytoplasmic membranes, but in recombinant vaccinia virus-infected cells it accumulated predominantly on the nuclear membrane.

MeSH Terms
Amino Acid Sequence Base Sequence Cytomegalovirus/genetics DNA, Viral/genetics Fluorescent Antibody Technique Genes, Viral Glycoproteins/genetics Herpesvirus 3, Human/genetics Herpesvirus 4, Human/genetics Humans Molecular Sequence Data Sequence Homology, Nucleic Acid Simplexvirus/genetics Vaccinia virus/genetics Viral Envelope Proteins/genetics Viral Proteins/genetics
Chemicals
DNA, Viral Glycoproteins Viral Envelope Proteins Viral Proteins glycoprotein H, herpes simplex virus type 1 glycoprotein gp3, varicella-zoster virus
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Cranage M P
Department of Pathology, University of Cambridge, England.
Smith G L
Bell S E
Hart H
Brown C
Bankier A T
Tomlinson P
Barrell B G
Minson T C
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.4.1416-1422.1988
Published
1988-04-00
Pages
1416-22
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253155
Subset
IM
Databases
GENBANK
M19882
External Links
PubMed source DOI: 10.1128/JVI.62.4.1416-1422.1988 PMC Full Text View journal details
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