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PMID: 1328840 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Reactivation of Mutator transposable elements of maize by ultraviolet light.

Molecular & general genetics : MGG ·Vol. 234 ·No. 3 ·1992-09-00 ·Pages 353-60

Walbot V

Abstract

After epigenetic loss of Mutator activity, the family of Mu elements in Zea mays becomes immobile and highly methylated; in addition, Mu9, the presumptive autonomous regulatory element, is transcriptionally silent and its copy number decreases in successive crosses to non-Mutator lines. Spontaneous reactivation, scored as restoration of somatic instability of potentially mutable alleles of Bronze-2, of such cryptic Mutator lines is rare, occurring with a frequency of about 10(-4). Irradiation of pollen with 254 nm ultraviolet light increases reactivation rate in the progeny kernels by up to 40-fold. Accompanying reactivation, the copy number of Mu9 elements increased, two-fold in one line and 20 to 40-fold in a second line. Reactivation may involve direct DNA damage or immediate physiological stress in the treated pollen.

MeSH Terms
DNA/metabolism,radiation effects DNA Transposable Elements/radiation effects Methylation Mutation Pollen/radiation effects Ultraviolet Rays Zea mays/genetics
Chemicals
DNA Transposable Elements DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Walbot V
Department of Biological Sciences, Stanford University, CA 94305-5020.
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32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00538694
Published
1992-09-00
Pages
353-60
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00538694
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