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PMID: 2555157 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The putative transposase of transposable element Ac from Zea mays L. interacts with subterminal sequences of Ac.

The EMBO journal ·Vol. 8 ·No. 11 ·1989-11-00 ·Pages 3177-85

Kunze R, Starlinger P

Abstract

The Ac-specific ORFa protein, overexpressed in a baculovirus system, specifically binds to several subterminal fragments of Ac. The 11 bp long inverted repeats of the transposable element are not bound by the ORFa protein. Major ORFa protein-binding sites were delineated on 60 and 70 bp long sequence segments that lie 100 bp inside of the 5' Ac terminus and 40 bp inside of the 3' terminus respectively. Within all strongly bound fragments, and particularly in these 60 or 70 bp long segments, the hexamer motif AAACGG is repeated several times in direct or inverted orientation. The ORFa protein binds to synthetic concatemers of this motif, whereas the mutant motif AAAGGG is not complexed. Methylation of the cytosine residues in the AAACGG motif and/or its complementary strand has pronounced effects: whereas one of the two hemimethylated sequences has a higher affinity to the ORFa protein than both unmethylated and holomethylated DNAs, the other hemimethylated DNA is virtually not complexed at all. The native ORFa protein binding sites are more complex than the AAACGG sequence: certain Ac and Ds1 fragments devoid of AAACGG motifs (but containing several similar sequences) are weakly bound by the ORFa protein.

MeSH Terms
Base Sequence Binding Sites DNA/genetics,metabolism DNA Mutational Analysis DNA Transposable Elements DNA-Binding Proteins/metabolism Electrophoresis, Agar Gel Methylation Molecular Sequence Data Nucleotidyltransferases/metabolism Precipitin Tests Repetitive Sequences, Nucleic Acid Restriction Mapping Transposases Zea mays/enzymology,genetics
Chemicals
DNA Transposable Elements DNA-Binding Proteins DNA Nucleotidyltransferases Transposases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kunze R
Institut für Genetik, Universität zu Köln, FRG.
Starlinger P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-11-00
Pages
3177-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401433
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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