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PMID: 2827168 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of base pairs in the outside end of insertion sequence IS50 that are needed for IS50 and Tn5 transposition.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 9118-22

Phadnis SH, Berg DE

Abstract

Short DNA sequences at ends of transposable elements are needed as sites for transposition. Previous deletion mapping showed that, in Tn5 and its component IS50 elements, these essential sites are about 19 base pairs long. To determine which positions are important in transposition, we made one or more sequence changes at each position in the IS50 outside (O) end and assayed the effects of these changes on transposition. Our results indicate that the specific base pairs at 18 of the 19 positions are important in transposition. A 9-base-pair segment in the O end corresponds to a binding site for the Escherichia coli DnaA protein. Comparisons of effects of mutations at different positions in this site, and also measurements of Tn5 transposition in dnaA- cells, indicate that DnaA protein participates in O-end-mediated transposition.

MeSH Terms
Bacterial Proteins/physiology Base Sequence Binding Sites DNA Mutational Analysis DNA Transposable Elements Escherichia coli/genetics Recombination, Genetic Structure-Activity Relationship
Chemicals
Bacterial Proteins DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Phadnis S H
Department of Microbiology, Washington University Medical School, St. Louis, MO 63110.
Berg D E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.9118
Published
1987-12-00
Pages
9118-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299703
Subset
IM
Grants
NIGMS NIH HHS · GM37138 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.24.9118 PMC Full Text View journal details
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