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PMID: 3038525 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA recognition by a new family of type I restriction enzymes: a unique relationship between two different DNA specificities.

The EMBO journal ·Vol. 6 ·No. 5 ·1987-05-00 ·Pages 1493-7

Price C, Shepherd JC, Bickle TA

Abstract

The DNA sequences recognized by the allelic type I restriction enzymes EcoR124 and EcoR124/3 were determined. EcoR124 recognizes 5'-GAA(N6)RTCG-3' and EcoR124/3 recognizes 5'-GAA(N7)RTCG-3'. These are typical of sequences recognized by type I recognition enzymes in that they consist of two specific domains separated by a non-specific spacer sequence. For these two enzymes, the specific sequences are identical but the length of the non-specific spacer is different. The specific domains of EcoR124/3 are thus 3.4 A further apart than those of EcoR124 and rotated with respect to each other through a further 36 degrees.

MeSH Terms
Base Sequence DNA DNA Restriction Enzymes/metabolism Deoxyribonucleases, Type I Site-Specific Methylation Plasmids Substrate Specificity
Chemicals
DNA DNA Restriction Enzymes endodeoxyribonuclease EcoR124-3I endodeoxyribonuclease EcoR124I Deoxyribonucleases, Type I Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Price C
Shepherd J C
Bickle T A
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40 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-05-00
Pages
1493-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553956
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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