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PMID: 6325176 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The EcoA restriction and modification system of Escherichia coli 15T-: enzyme structure and DNA recognition sequence.

The EMBO journal ·Vol. 3 ·No. 3 ·1984-03-00 ·Pages 575-9

Suri B, Shepherd JC, Bickle TA

Abstract

The EcoA restriction enzyme from Escherichia coli 15T- has been isolated. It proves to be an unusual enzyme, clearly related functionally to the classical type I restriction enzymes. The basic enzyme is a two subunit modification methylase. Another protein species can be purified which by itself has no enzymatic activities but which converts the modification methylase to an ATP and S-adenosylmethionine-dependent restriction endonuclease. The DNA recognition sequence of EcoA has an overall structure that is very similar to previously determined type I sequences. It is: 5'-GAGNNNNNNNGTCA-3' 3'-CTCNNNNNNNCAGT-5' where N can be any nucleotide. Modification methylates the adenosyl residue in the specific trinucleotide and the adenosyl residue in the lower strand of the specific tetranucleotide.

MeSH Terms
Base Sequence DNA Restriction Enzymes/isolation & purification,metabolism Deoxyribonucleases, Type I Site-Specific Escherichia coli/enzymology,genetics Methylation Molecular Weight Substrate Specificity
Chemicals
DNA Restriction Enzymes endodeoxyribonuclease EcoAI Deoxyribonucleases, Type I Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Suri B
Shepherd J C
Bickle T A
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-03-00
Pages
575-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557390
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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