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PMID: 353810 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the recognition site for the restriction-modification enzyme of Escherichia coli B.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 5 ·1978-05-00 ·Pages 2266-70

Ravetch JV, Horiuchi K, Zinder ND

Abstract

The nucleotide sequence of the recognition site for the restriction-modification enzyme of Escherichia coli B (SB site) has been determined. The recognition site is a 15-nucleotide sequence consisting of the trimer 5'TGA3', followed by an 8-nucleotide domain of variable sequence, which in turn is followed by tetramer 5'TGCT3'. The sequence has no 2-fold rotational symmetry. Single base changes in the constant nucleotide domains result in the loss of sensitivity to both restriction and modification. Our data are also consistent with modification occurring by methylation of two adenine residues per SB site: one on the adenine of the trimer 5'TGA3' and the other on the complementary strand on the adenine complementary to the first thymine of the tetramer 5'TGCT3'. All nine independently isolated spontaneous mutants at the SB1 site of bacteriophage f1 are caused by a G-to-T transversion. Mutations at the SB2 site are caused by various single base changes.

MeSH Terms
Adenine/analogs & derivatives Base Sequence Coliphages/genetics DNA Restriction Enzymes/metabolism DNA, Viral/metabolism Escherichia coli/enzymology Methylation Substrate Specificity
Chemicals
DNA, Viral DNA Restriction Enzymes Adenine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ravetch J V
Horiuchi K
Zinder N D
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.5.2266
Published
1978-05-00
Pages
2266-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392533
Subset
IM
Databases
GENBANK
M10677, M10744
External Links
PubMed source DOI: 10.1073/pnas.75.5.2266 PMC Full Text View journal details
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