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PMID: 6320124 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The nucleotide recognized by the Escherichia coli A restriction and modification enzyme.

Nucleic acids research ·Vol. 12 ·No. 2 ·1984-01-25 ·Pages 887-99

Kröger M, Hobom G

Abstract

The nucleotide recognition sequence for the restriction-modification enzyme of Escherichia coli A (EcoA) has been determined to be GAG-7N-GTCA. This sequence is fairly similar, but distinctly different from the two other type I restriction enzyme recognition sites known for E. coli B and E. coli K12, respectively. N6-adenosine methylation has been observed at nucleotide positions 2 and 12 within that sequence after modification by EcoA. As a reference point for mapping the single EcoA site in lambda, the position of lambda point mutation Oam29 has been determined also.

MeSH Terms

Base Sequence DNA Restriction Enzymes/metabolism Deoxyribonucleases, Type I Site-Specific Escherichia coli/enzymology Plasmids Substrate Specificity

Chemicals

DNA Restriction Enzymes endodeoxyribonuclease EcoAI Deoxyribonucleases, Type I Site-Specific

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kröger M

Hobom G

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.2.887

Published

1984-01-25

Pages

887-99

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318543

Subset

External Links

PubMed source DOI: 10.1093/nar/12.2.887 PMC Full Text View journal details

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