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PMID: 2987794 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence recognised by the Escherichia coli D type I restriction and modification enzyme.

Nucleic acids research ·Vol. 13 ·No. 2 ·1985-01-25 ·Pages 389-99

Nagaraja V, Stieger M, Nager C, Hadi SM, Bickle TA

Abstract

A type I restriction endonuclease from a new isolate of Escherichia coli (E. coli E166) has been purified and characterised. The enzyme, EcoD, has a recognition sequence similar in overall structure to the previously determined type I enzyme sequences, an exception being that it is degenerate. The sequence is 5'-T-T-A-N-N-N-N-N-N-N-G-T-C-Y-3' 3'-A-A-T-N-N-N-N-N-N-N-C-A-G-R-5' where Y is a pyrimidine, R is a purine and N can be any nucleotide. The enzyme methylates adenosyl residues in both strands of the DNA that are separated by ten base pairs, suggesting that the enzyme interacts with DNA along one face of the helix making contacts in two successive major grooves.

MeSH Terms
Base Sequence DNA Restriction Enzymes/isolation & purification,metabolism Deoxyribonucleases, Type I Site-Specific Escherichia coli/enzymology Methylation Substrate Specificity
Chemicals
DNA Restriction Enzymes endodeoxyribonuclease EcoDI Deoxyribonucleases, Type I Site-Specific
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nagaraja V
Stieger M
Nager C
Hadi S M
Bickle T A
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.2.389
Published
1985-01-25
Pages
389-99
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341003
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.2.389 PMC Full Text View journal details
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