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PMID: 6096829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete sequence of pSC101.

Nucleic acids research ·Vol. 12 ·No. 24 ·1984-12-21 ·Pages 9415-26

Bernardi A, Bernardi F

Abstract

We have completed the pSC101 sequence. The coding capacities of the newly sequenced regions show the presence of two large open reading frames close to the oriT region. Their size and localization suggest that these polypeptide chains could be involved in the transfer process of pSC101.

MeSH Terms

Base Sequence Conjugation, Genetic DNA Restriction Enzymes DNA, Bacterial/genetics Hydrogen Bonding Nucleic Acid Conformation Plasmids R Factors Salmonella/genetics

Chemicals

DNA, Bacterial DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bernardi A

Bernardi F

References (31)

31 references, click to expand

Primary structure of the essential replicon of the plasmid pSC101.
Proc Natl Acad Sci U S A. 1983 Nov;80(21):6557-61 PMID: 6579542
The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101.
Nucleic Acids Res. 1983 Aug 25;11(16):5645-59 PMID: 6310509
A 37 X 10(3) molecular weight plasmid-encoded protein is required for replication and copy number control in the plasmid pSC101 and its temperature-sensitive derivative pHS1.
J Mol Biol. 1984 May 25;175(3):331-48 PMID: 6327996
Atypical deletions generated by mutated IS102 elements.
Mol Gen Genet. 1984;195(3):452-8 PMID: 6088945
Molecular and genetic studies of an R factor system consisting of independent transfer and drug resistance plasmids.
J Bacteriol. 1973 Nov;116(2):699-709 PMID: 4583247
Construction of biologically functional bacterial plasmids in vitro.
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
Specificity of substrate recognition by the EcoRI restriction endonuclease.
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310-4 PMID: 242001
Increase in conjugational transmission frequency of nonconjugative plasmids.
Science. 1977 Apr 8;196(4286):186-7 PMID: 322280
Revised interpretation of the origin of the pSC101 plasmid.
J Bacteriol. 1977 Nov;132(2):734-7 PMID: 334752
Replication of plasmid pSC101 in Escherichia coli K12: requirement for dnaA function.
Mol Gen Genet. 1977 Sep 9;154(3):225-30 PMID: 337104
A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus.
Gene. 1977 Jul;1(5-6):323-9 PMID: 590742
Massive airway hemorrhage after transtracheal aspiration.
South Med J. 1978 Mar;71(3):325-7 PMID: 628856
Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
Gene. 1977;2(2):75-93 PMID: 344136
A general method for the purification of restriction enzymes.
Nucleic Acids Res. 1978 Jul;5(7):2373-80 PMID: 673857
ColE1 plasmid mobility and relaxation complex.
Nature. 1978 Jul 20;274(5668):259-61 PMID: 210387
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Insertion element IS102 resides in plasmid pSC101.
J Bacteriol. 1980 Oct;144(1):131-40 PMID: 6252187
Location of two relaxation nick sites in R6K and single sites in pSC101 and RSF1010 close to origins of vegetative replication: implication for conjugal transfer of plasmid deoxyribonucleic acid.
J Bacteriol. 1980 Dec;144(3):923-32 PMID: 6254952
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Organization of chimeras between filamentous bacteriophage f1 and plasmid pSC101.
J Mol Biol. 1980 Dec 15;144(3):247-65 PMID: 6265643
Complete sequence of an IS element present in pSC101.
Nucleic Acids Res. 1981 Jun 25;9(12):2905-11 PMID: 6269064
A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322.
Gene. 1981 Dec;16(1-3):21-6 PMID: 6282692
Plasmid ColE1 conjugal mobility: the nature of bom, a region required in cis for transfer.
Mol Gen Genet. 1982;185(2):344-51 PMID: 7045589
pEMBL: a new family of single stranded plasmids.
Nucleic Acids Res. 1983 Mar 25;11(6):1645-55 PMID: 6300771
Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1830-4 PMID: 6340110
Location and nucleotide sequence of the transfer origin of the broad host range plasmid RK2.
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3595-8 PMID: 6304722
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
Revised sequence of the tetracycline-resistance gene of pBR322.
Gene. 1983 May-Jun;22(2-3):277-80 PMID: 6307828
Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101.
Gene. 1983 Oct;24(2-3):309-15 PMID: 6357952

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.24.9415

Published

1984-12-21

Pages

9415-26

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320470

Subset

Databases

GENBANK

X01654

External Links

PubMed source DOI: 10.1093/nar/12.24.9415 PMC Full Text View journal details

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