Replication of plasmid pSC101 in Escherichia coli K12: requirement for dnaA function.

Molecular & general genetics : MGG ·Vol. 154 ·No. 3 ·1977-09-09 ·Pages 225-30

Hasunuma K, Sekiguchi M

Abstract

Replication of pSC101 was analyzed by using DNA-DNA hybridization and alkaline sucrose gradient centrifugation. Mutants of the dnaA gene were tested for their capacity to replicate pSC101 DNA at a non-permissive temperature. Only a small amount of radioactive precursor was incorporated into pSC101 DNA in dnaA mutants at 42 degrees C whereas active incorporation into plasmid DNA took place in dnaA+ strain under the same conditions. The effect of the dnaA mutation was grater on plasmid DNA synthesis than on host chromosomal DNA synthesis. The numbers of copies of pSC101 per chromosome in wild type and dnaA strains, grown at 30 degrees C, were about 8 and 2, respectively. These results indicate that the dna A gene product is required for the replication of pSC101 DNA.

MeSH Terms

Chromosomes, Bacterial/metabolism DNA Replication DNA, Bacterial/genetics Escherichia coli/genetics,ultrastructure Hot Temperature Mutation R Factors Transcription, Genetic

Chemicals

DNA, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hasunuma K

Sekiguchi M

References (15)

15 references, click to expand

Construction of biologically functional bacterial plasmids in vitro.
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
A membrane-filter technique for the detection of complementary DNA.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
General method for the isolation of plasmid deoxyribonucleic acid.
J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
A quantitative assay for DNA-DNA hybrids using membrane filters.
Biochem Biophys Res Commun. 1966 Aug 23;24(4):554-8 PMID: 5338489
Sensitivity of Escherichia coli to viral nucleic acid. VI. Capacity of dna mutants and DNA polymerase-less mutants for multiplication of phiA and phiX 174.
Mol Gen Genet. 1973 Mar 27;122(1):15-22 PMID: 4573259
Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
Indirect selection of bacterial plasmids lacking identifiable phenotypic properties.
J Bacteriol. 1975 Oct;124(1):225-31 PMID: 1100600
Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4 PMID: 4610569
Escherichia coli mutants temperature-sensitive for DNA synthesis.
Mol Gen Genet. 1971;113(3):273-84 PMID: 4946856
Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.
Mol Gen Genet. 1975;136(4):273-89 PMID: 16094996
Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons.
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4556-60 PMID: 4612523
The influence of DNA A and DNA C mutations on the initiation of plasmid DNA replication.
Biochem Biophys Res Commun. 1973 Apr 16;51(4):1000-7 PMID: 4574153
Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.
Cold Spring Harb Symp Quant Biol. 1968;33:677-93 PMID: 4892005
Isolation of temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine.
J Bacteriol. 1976 Sep;127(3):1561-3 PMID: 783149
Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants.
Proc Natl Acad Sci U S A. 1973 May;70(5):1293-7 PMID: 4576014

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00571277

Published

1977-09-09

Pages

225-30

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00571277 免费全文

Analysis Services

Replication of plasmid pSC101 in Escherichia coli K12: requirement for dnaA function.

Contact

WeChat Official Account