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PMID: 6088945 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Atypical deletions generated by mutated IS102 elements.

Molecular & general genetics : MGG ·Vol. 195 ·No. 3 ·1984-00-00 ·Pages 452-8

Bernardi F, Bernardi A

Abstract

The element IS102 potentially codes for two polypeptide chains. We have introduced several mutations in the larger one near the COOH terminus and determined the residual ability of the mutated elements to generate deletions in order to assign a role to this polypeptide chain. We show that in these elements, deletions still occur, although at a reduced level, but that in all cases examined so far the ends of the element are no longer recognized as the fixed endpoint of IS-mediated deletions, even though some other structural features of normal deletions formation are still present.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence Chromosome Mapping DNA Restriction Enzymes DNA Transposable Elements DNA, Viral/genetics Genes, Viral Mutation Plasmids
Chemicals
DNA Transposable Elements DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bernardi F
Bernardi A
References (28)
28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00341446
Published
1984-00-00
Pages
452-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00341446
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