Home LiteratureArticle Details
PMID: 6276699 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new type of IS1-mediated deletion.

Molecular & general genetics : MGG ·Vol. 184 ·No. 2 ·1981-00-00 ·Pages 300-7

Sommer H, Schumacher B, Saedler H

Abstract

Genetical tests and DNA sequence analysis revealed that the mechanism of formation of IS1-induced type I and type II deletions differs. IS1-mediated type II deletions occur at the termini of the integrated element and do not remove the element. This process is independent of the cellular recA system and does not involve DNA sequence homology. Conversely, the formation of IS1-induced type I deletions differs substantially. They require recA gene product, small DNA sequence duplications and a topological arrangement of the DNA molecule to allow alignment of duplications.

MeSH Terms
Base Sequence Chromosome Deletion Coliphages/genetics DNA Transposable Elements DNA, Bacterial/analysis DNA, Viral/analysis Escherichia coli/genetics Genes, Bacterial Plasmids Recombination, Genetic
Chemicals
DNA Transposable Elements DNA, Bacterial DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sommer H
Schumacher B
Saedler H
References (14)
14 references, click to expand
  1. Development of a system useful for studying the formation of unstable alleles of IS2.
    Mol Gen Genet. 1979 May 23;173(1):15-21 PMID: 381839
  2. Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.
    J Mol Biol. 1978 Dec 25;126(4):847-57 PMID: 370408
  3. Nucleotide sequence of the transposable DNA-element IS2.
    Nucleic Acids Res. 1979 Mar;6(3):1111-22 PMID: 375194
  4. IS1-mediated DNA rearrangements.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:93-8 PMID: 6271496
  5. Insertion mutations in the control region of the galactose operon of E. coli. II. Physical characterization of the mutations.
    Mol Gen Genet. 1972;115(3):266-76 PMID: 4555680
  6. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
    J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
  7. Three Tn10-associated excision events: relationship to transposition and role of direct and inverted repeats.
    Cell. 1981 Jan;23(1):215-27 PMID: 6260376
  8. Nucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):106-10 PMID: 319453
  9. Integration of IS3 into IS2 generates a short sequence duplication.
    Mol Gen Genet. 1979;177(1):85-9 PMID: 395417
  10. IS1 is involved in deletion formation in the gal region of E. coli K12.
    Mol Gen Genet. 1975;137(1):17-28 PMID: 1101028
  11. IS2-61 and IS2-611 arise by illegitimate recombination from IS2-6.
    Mol Gen Genet. 1979 Oct 3;176(2):233-8 PMID: 393955
  12. Nucleotide sequence of an insertion element, IS1.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):615-9 PMID: 273224
  13. 0 degree mutations in the galactose operon in E. coli. II. Physiological characterization.
    Mol Gen Genet. 1967;100(2):190-202 PMID: 4869990
  14. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00272921
Published
1981-00-00
Pages
300-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00272921
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2