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PMID: 6310509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5645-59

Churchward G, Linder P, Caro L

Abstract

The nucleotide sequence of 1100bp around the origin of replication of the pSC101 plasmid has been determined. This segment of DNA is capable of replication in the presence of a helper plasmid. The sequence data reveal similarities between pSC101 and several other replicons. The origin of replication contains three direct repeats of an 18bp sequence associated with a segment exceptionally rich in A-T base pairs. A promotor that probably directs transcription of a gene encoding an essential plasmid replication function is associated with a region of extensive potential secondary structure. The sequence presented here includes the sequence of the par region involved in partitioning of plasmids at cell division.

MeSH Terms
Base Composition Base Sequence Cloning, Molecular Codon DNA Replication DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Mutation Nucleic Acid Conformation Operon Plasmids
Chemicals
Codon DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Churchward G
Linder P
Caro L
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47 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.16.5645
Published
1983-08-25
Pages
5645-59
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326303
Subset
IM
Databases
GENBANK
K00042, K00626, K00828, X00863, X04053
External Links
PubMed source DOI: 10.1093/nar/11.16.5645 PMC Full Text View journal details
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