Home LiteratureArticle Details
PMID: 6156450 Published · ppublish English Journal Article

Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 2450-4

Itoh T, Tomizawa J

Abstract

A plasmid that consists of an 812-base-pair segment containing the replication origin of plasmid ColE1 and of a 1240-base-pair segment containing a beta-lactamase gene has been constructed. The plasmid DNA has three principal sites where transcription is initiated in vitro. One is located in the ColE1 segment 555 nucleotides upstream from the origin. Most transcription from this site extends past the origin; some of the transcripts form hybrids spontaneously with the template at their 3' portions. Cleavage of these transcripts by RNase H generates 3' termini at the origin region. When DNA polymerase I is included in the reaction along with RNA polymerase and RNase H, dAMP or dCMP is added directly onto the cleaved RNA molecules, most of which retain the intact 5' terminus. The addition of a deoxyribonucleotide to the cleaved RNA can be regarded as the first step of ColE1 DNA synthesis. Once it has served as a primer, the RNA is eliminated from the product by RNase H.

MeSH Terms
Bacteriocin Plasmids DNA Polymerase I/metabolism DNA Replication DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics Nucleic Acid Precursors/metabolism Plasmids RNA, Bacterial/metabolism Ribonucleases/metabolism Transcription, Genetic
Chemicals
Nucleic Acid Precursors RNA, Bacterial DNA-Directed RNA Polymerases DNA Polymerase I Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Itoh T
Tomizawa J
References (31)
31 references, click to expand
  1. Integration-negative mutants of bacteriophage lambda.
    J Mol Biol. 1968 Feb 14;31(3):487-505 PMID: 5637199
  2. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.
    J Biol Chem. 1969 Jun 10;244(11):2996-3008 PMID: 4890762
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid.
    J Biol Chem. 1971 Aug 25;246(16):5120-39 PMID: 4936723
  5. The sequence of 5 s ribosomal ribonucleic acid.
    J Mol Biol. 1968 Jun 28;34(3):379-412 PMID: 4938553
  6. A dihydroxyboryl-substituted methacrylic polymer for the column chromatographic separation of mononucleotides, oligonucleotides, and transfer ribonucleic acid.
    Biochemistry. 1973 Feb 27;12(5):932-8 PMID: 4568770
  7. Isolation and characterization of an endonuclease from Escherichia coli specific for ribonucleic acid in ribonucleic acid-deoxyribonucleic acid hybrid structures.
    J Biol Chem. 1973 Sep 10;248(17):5914-21 PMID: 4580046
  8. Replication of colicin E1 plasmid DNA in cell extracts.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):802-6 PMID: 4595573
  9. Replication of colicin E1 plasmid DNA added to cell extracts.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1050-4 PMID: 1093162
  10. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
  11. Selective inhibition of in vitro DNA synthesis dependent on phiX174 compared with fd DNA. I. Protein requirements for selective inhibition.
    J Biol Chem. 1977 Apr 25;252(8):2524-33 PMID: 140166
  12. Origin of replication of colicin E1 plasmid DNA.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1865-9 PMID: 325558
  13. New rapid gel sequencing method for RNA.
    Nature. 1977 Oct 27;269(5631):833-6 PMID: 927512
  14. Origin of replication of pBR345 plasmid DNA.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5265-9 PMID: 271952
  15. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  16. Nucleotide sequence of cro, cII and part of the O gene in phage lambda DNA.
    Nature. 1978 Mar 30;272(5652):410-4 PMID: 264238
  17. Ribonucleotide-deoxyribonucleotide linkages at the origin of DNA replication of colicin E1 plasmid.
    J Mol Biol. 1978 Mar 25;120(1):137-43 PMID: 642005
  18. Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1270-3 PMID: 274717
  19. Enzymatic oligoribonucleotide synthesis with T4 RNA ligase.
    Biochemistry. 1978 May 30;17(11):2069-76 PMID: 667012
  20. Structure and replication of the colicin E1 plasmid.
    Curr Top Microbiol Immunol. 1978;83:93-156 PMID: 365473
  21. Refined molecular weights for phage, viral and ribosomal RNA.
    Gene. 1978 Jul;3(4):353-7 PMID: 365689
  22. The structure of a transcriptional unit on colicin E1 plasmid.
    Eur J Biochem. 1979 Jul;97(2):435-43 PMID: 380993
  23. Initiation of DNA synthesis in Escherichia coli.
    Annu Rev Biochem. 1979;48:999-1034 PMID: 382998
  24. Initiation of replication of plasmid ColE1 DNA by RNA polymerase, ribonuclease H, and DNA polymerase I.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:409-17 PMID: 225109
  25. Genetic and physical studies on the replication of ColE1-type plasmids.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:69-76 PMID: 383386
  26. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  27. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.
    Mol Gen Genet. 1979 May 4;172(2):151-9 PMID: 384144
  28. Rapid RNA sequencing: nucleases from Staphylococcus aureus and Neurospora crassa discriminate between uridine and cytidine.
    Nucleic Acids Res. 1979 Aug 10;6(11):3481-90 PMID: 158747
  29. The complete nucleotide sequence of the ribosomal 16-S RNA from Excherichia coli. Experimental details and cistron heterogeneities.
    Eur J Biochem. 1979 Oct 15;100(2):399-410 PMID: 389624
  30. Nucleotide sequence of the region required for maintenance of colicin E1 plasmid.
    Mol Gen Genet. 1979 Oct 3;176(2):161-70 PMID: 393952
  31. Organization of the recA gene of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):313-7 PMID: 6244554
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.5.2450
Published
1980-05-00
Pages
2450-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349417
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.5.2450 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2