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PMID: 393952 Published · ppublish English Journal Article

Nucleotide sequence of the region required for maintenance of colicin E1 plasmid.

Molecular & general genetics : MGG ·Vol. 176 ·No. 2 ·1979-10-03 ·Pages 161-70

Ohmori H, Tomizawa J

Abstract

Plasmids carrying various portions of colicin E1 plasmid (ColE1) DNA have been isolated in an attempt to determine the regions of ColE1 DNA which are required for maintenance of the plasmid in bacteria. To construct the plasmids, the DNA of a ColE1 derivative that contains a gene which controls ampicillin resistance was cleaved by the restriction endonuclease HaeII. The digestion products were joined by T4 DNA ligase and then used to transform bacteria to ampicillin resistance. The plasmid derivatives obtained in this way were always composed of certain HaeII segments. These contain approximately 10% of the ColE1 genome and include the origin of replication of ColE1. We presume that the region of ColE1 which is common to all these derivatives is required for maintenance of the plasmid. After a description of these results, the nucleotide sequence of this region is presented, and possible roles of the region in plasmid replication and maintenance are discussed.

MeSH Terms
Bacteriocin Plasmids Base Sequence DNA Replication DNA, Bacterial/genetics Escherichia coli/genetics Plasmids
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ohmori H
Tomizawa J
References (32)
32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00273210
Published
1979-10-03
Pages
161-70
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
V00270
External Links
PubMed source DOI: 10.1007/BF00273210
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