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PMID: 271952 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Origin of replication of pBR345 plasmid DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5265-9

Bolivar F, Betlach MC, Heyneker HL, Shine J, Rodriguez RL, Boyer HW

Abstract

A small (approximately 1100 base pairs) ColE1-type plasmid, pBR345, was constructed from plasmid pMB1 by a series of in vitro recombinant manipulations. Approximately 9% of the supercoiled pBR345 DNA obtained from cultures amplified with chloramphenicol appears to be replicative intermediates with replicating "eye" structures of uniform size. Results obtained from electron microscopy and biochemical analyses have enabled us to localize the origin of replication at the same position as that reported for ColE1. A sequence of 420 nucleotides surrounding this origin has been determined. A comparison between this sequence and the one determined for the origin of replication of ColE1 is presented.

MeSH Terms
Base Sequence Chromosome Mapping Colicins DNA Replication DNA Restriction Enzymes DNA, Bacterial/biosynthesis DNA, Superhelical/biosynthesis Microscopy, Electron Plasmids
Chemicals
Colicins DNA, Bacterial DNA, Superhelical DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bolivar F
Betlach M C
Heyneker H L
Shine J
Rodriguez R L
Boyer H W
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5265
Published
1977-12-00
Pages
5265-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431677
Subset
IM
Databases
GENBANK
M10848
External Links
PubMed source DOI: 10.1073/pnas.74.12.5265 PMC Full Text View journal details
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