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PMID: 1330541 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of a negative regulatory domain in the human papillomavirus type 18 promoter: interaction with the transcriptional repressor YY1.

The EMBO journal ·Vol. 11 ·No. 12 ·1992-12-00 ·Pages 4607-17

Bauknecht T, Angel P, Royer HD, zur Hausen H

Abstract

The human papillomavirus type 18 (HPV-18) promoter contains a TPA responsive element (TRE) which confers TPA responsiveness on a heterologous promoter. In the context of the HPV-18 promoter, however, this AP-1 site is inactive. We have identified a negative regulatory domain in the HPV-18 promoter which represses the constitutive and TPA-induced AP-1 activity. This negative regulatory sequence has been mapped to 44 nucleotides (OL13). We identified this element as a transcriptional silencer based on its ability to interfere with transcriptional initiation. This HPV-18 silencer domain was narrowed down further to 23 nucleotides, the OL13B element, which bears similarity to three other silencer sequences, present in the mouse N-ras gene upstream regulatory region, the mouse albumin gene enhancer and the adeno-associated virus P5 promoter. The transcriptional repressor protein YY1, which negatively regulates the P5 promoter, binds to the HPV-18 silencer with high affinity. Mutation of the YY1 binding site leads to an enhanced activity of the HPV-18 promoter, strongly suggesting that YY1 plays an important role in controlling HPV-18 early gene expression.

MeSH Terms
Base Sequence Binding Sites Cells, Cultured Chloramphenicol O-Acetyltransferase/genetics DNA DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Erythroid-Specific DNA-Binding Factors Genes, Viral HeLa Cells Humans Molecular Sequence Data Mutation Papillomaviridae/genetics Polymerase Chain Reaction Promoter Regions, Genetic Proto-Oncogene Proteins c-jun/metabolism Tetradecanoylphorbol Acetate/pharmacology Transcription Factors Transcription, Genetic YY1 Transcription Factor
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Proto-Oncogene Proteins c-jun Transcription Factors YY1 Transcription Factor YY1 protein, human DNA Chloramphenicol O-Acetyltransferase Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bauknecht T
Deutsches Krebsforschungszentrum Heidelberg, Germany.
Angel P
Royer H D
zur Hausen H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-12-00
Pages
4607-17
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557037
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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