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PMID: 1946405 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of a candidate repressor/activator, NF-E1 (YY-1, delta), that binds to the immunoglobulin kappa 3' enhancer and the immunoglobulin heavy-chain mu E1 site.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 21 ·1991-11-01 ·Pages 9804-8

Park K, Atchison ML

Abstract

We have determined that the developmental control of immunoglobulin kappa 3' enhancer (kappa E3') activity is the result of the combined influence of positive- and negative-acting elements. We show that a central core in the kappa E3' enhancer is active at the pre-B-cell stage but is repressed by flanking negative-acting elements. The negative-acting sequences repress enhancer activity in a position- and orientation-independent manner at the pre-B-cell stage. We have isolated a human cDNA clone encoding a zinc finger protein (NF-E1) that binds to the negative-acting segment of the kappa E3' enhancer. This protein also binds to the immunoglobulin heavy-chain enhancer mu E1 site. NF-E1 is encoded by the same gene as the YY-1 protein, which binds to the adeno-associated virus P5 promoter. NF-E1 is also the human homologue of the mouse delta protein, which binds to ribosomal protein gene promoters. The predicted amino acid sequence of this protein contains features characteristic of transcriptional activators as well as transcriptional repressors. Cotransfection studies with this cDNA indicate that it can repress basal promoter activity. The apparent dual function of this protein is discussed.

MeSH Terms
Amino Acid Sequence B-Lymphocytes/physiology Base Sequence Cloning, Molecular DNA/genetics DNA-Binding Proteins/genetics,metabolism Enhancer Elements, Genetic Erythroid-Specific DNA-Binding Factors Gene Expression Regulation Genes, Immunoglobulin Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin kappa-Chains/genetics Molecular Sequence Data Nuclear Proteins/genetics,isolation & purification Oligonucleotides/chemistry Recombinant Proteins/metabolism Regulatory Sequences, Nucleic Acid Repressor Proteins/genetics,isolation & purification Transcription Factors/genetics,isolation & purification Transcription, Genetic Zinc Fingers
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Immunoglobulin Heavy Chains Immunoglobulin kappa-Chains Nuclear Proteins Oligonucleotides Recombinant Proteins Repressor Proteins Transcription Factors DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Park K
Department of Animal Biology, University of Pennsylvania, School of Veterinary Medicine, Philadelphia 19104.
Atchison M L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.21.9804
Published
1991-11-01
Pages
9804-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52809
Subset
IM
Grants
NIGMS NIH HHS · R01 GM42415 · United States
Databases
GENBANK
M76541, M80229, M80230, M80231, M80232, S67589, S67595, S67599, S68142, S68244
External Links
PubMed source DOI: 10.1073/pnas.88.21.9804 PMC Full Text 免费全文 View journal details
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