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PMID: 1366393 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase.

Applied microbiology and biotechnology ·Vol. 32 ·No. 4 ·1990-01-00 ·Pages 443-8

Thierbach G, Kalinowski J, Bachmann B, Pühler A

Abstract

The Corynebacterium glutamicum/Escherichia coli shuttle vector plasmid pZ1 was used to clone the S-(2-aminoethyl)-D,L-cysteine (AEC)-resistance gene from a lysine-excreting, AEC-resistant strain of C. glutamicum, the aspartokinase activity of which was released from feedback inhibition by mixtures of lysine and threonine or AEC and threonine respectively. A recombinant plasmid designated pCS2 carrying a 9.9-kb chromosomal insert that conferred AEC resistance and the ability to excrete lysine to its host was isolated. The aspartokinase activity of the pCS2-carrying strain was resistant towards inhibition by mixtures of lysine and threonine or AEC and threonine respectively. By deletion analysis the DNA region conferring AEC resistance to the host and feedback resistance to its aspartokinase activity could be confined to a 1.2-kb DNA fragment.

MeSH Terms
Aspartate Kinase/genetics,pharmacology Biotechnology Cloning, Molecular Corynebacterium/drug effects,genetics Cysteine/analogs & derivatives,pharmacology DNA, Bacterial/genetics Drug Resistance, Microbial/genetics Feedback Restriction Mapping
Chemicals
DNA, Bacterial S-2-aminoethyl cysteine Aspartate Kinase Cysteine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thierbach G
Degussa AG/ASTA Pharma AG, Bielefeld, Federal Republic of Germany.
Kalinowski J
Bachmann B
Pühler A
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Article Info
Journal
Applied microbiology and biotechnology
Abbr.
Appl Microbiol Biotechnol
ISSN
0175-7598
DOI
10.1007/BF00903780
Published
1990-01-00
Pages
443-8
Language
English
Region
Germany
NLM ID
8406612
External Links
PubMed source DOI: 10.1007/BF00903780 View journal details
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