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PMID: 3921526 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning vector system for Corynebacterium glutamicum.

Journal of bacteriology ·Vol. 162 ·No. 2 ·1985-05-00 ·Pages 591-7

Yoshihama M, Higashiro K, Rao EA, Akedo M, Shanabruch WG, Follettie MT, Walker GC, Sinskey AJ

Abstract

A protoplast transformation system has been developed for Corynebacterium glutamicum by using a C. glutamicum-Bacillus subtilis chimeric vector. The chimera was constructed by joining a 3.0-kilobase cryptic C. glutamicum plasmid and the B. subtilis plasmid pBD10. The neomycin resistance gene on the chimera, pHY416, was expressed in C. glutamicum, although the chloramphenicol resistance gene was not. The various parameters in the transformation protocol were analyzed separately and optimized. The resulting transformation system is simple and routinely yields 10(4) transformants per microgram of plasmid DNA.

MeSH Terms
Bacillus subtilis/genetics Cloning, Molecular/methods Corynebacterium/genetics Gene Expression Regulation Genetic Vectors Plasmids Transformation, Genetic
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Yoshihama M
Higashiro K
Rao E A
Akedo M
Shanabruch W G
Follettie M T
Walker G C
Sinskey A J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.162.2.591-597.1985
Published
1985-05-00
Pages
591-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218889
Subset
IM
Grants
NCI NIH HHS · T32-CA-09258-06 · United States
External Links
PubMed source DOI: 10.1128/jb.162.2.591-597.1985 PMC Full Text View journal details
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