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PMID: 418411 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Molecular cloning of genetically active fragments of Bacillus DNA in Bacillus subtilis and properties of the vector plasmid pUB110.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 3 ·1978-03-00 ·Pages 1423-7

Keggins KM, Lovett PS, Duvall EJ

Abstract

Plasmid pUB110 (approximately 2.8 x 10(6) daltons), originally detected in Staphylococcus aureus, specifies resistance to neomycin and has been transformed into Bacillus subtilis 168, In B. subtilis, pUB110 is stably maintained at about 50 copies per chromosome and renders the host resistant to neomycin sulfate at 5 microgram/ml. pUB110 isolated from B. subtilis transforms Rec+ and recE4-containing strains of B. subtilis at frequencies greater than or equal to 10(3) transformants per microgram of plasmid. pUB110 was transferred by PBS1 or SP10 transduction from B. subtilis to strains of B. pumilus and B. licheniformis. pUB110 is compatible with each of four previously described Bacillus plasmids, including pPL576, pPL10, pPL7065, and pPL2. pUB110 contains a single EcoR1-sensitive site and was used as vector to clone DNA fragments that complement the trpC2 mutation in B. subtilis 168 from EcoR1 digests of the chromosome DNA isolated from B. pumilus strains NRRL B-3275 and NRS576, B. licheniformis strains 9945A and 749C, and B. subtilis 168. Genetic and physical properties of each of the constructed Trp derivatives of pUB110 are described.

MeSH Terms
Bacillus/genetics Bacillus subtilis/genetics DNA Restriction Enzymes DNA, Bacterial/genetics DNA, Recombinant Drug Resistance, Microbial Genetic Complementation Test Neomycin/pharmacology Plasmids Transduction, Genetic Transformation, Genetic Tryptophan/genetics
Chemicals
DNA, Bacterial DNA, Recombinant Tryptophan DNA Restriction Enzymes Neomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Keggins K M
Lovett P S
Duvall E J
References (29)
29 references, click to expand
  1. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
    Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
  2. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
    J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352
  3. TRANSDUCTION OF BACILLUS LICHENIFORMIS AND BACILLUS SUBTILIS BY EACH OF TWO PHAGES.
    J Bacteriol. 1963 Sep;86:452-61 PMID: 14066421
  4. New temperate bacteriophage for Bacillus subtilis, rho 11.
    J Virol. 1976 Nov;20(2):509-19 PMID: 62060
  5. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680-2 PMID: 404641
  6. Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.
    J Bacteriol. 1977 Jul;131(1):374-7 PMID: 406259
  7. Isolation and characterization of four plasmids from Bacillus subtilis.
    J Bacteriol. 1977 Mar;129(3):1487-94 PMID: 403179
  8. Isolation and characterization of four types of plasmids from Bacillus subtilis (natto).
    J Bacteriol. 1977 Aug;131(2):699-701 PMID: 407218
  9. Host function specified by Bacillus pumilus plasmid pPL7065.
    Antimicrob Agents Chemother. 1977 Sep;12(3):435-7 PMID: 907334
  10. Enzymes of the tryptophan operon of Bacillus subtilis.
    Biochem Biophys Res Commun. 1969 Jun 27;35(6):838-44 PMID: 4978220
  11. Molecular structure of bacterial plasmids.
    Bacteriol Rev. 1972 Sep;36(3):361-405 PMID: 4345849
  12. Genetic characterization of recombination-deficient mutants of Bacillus subtilis.
    J Bacteriol. 1974 Feb;117(2):488-93 PMID: 4272775
  13. Intergenotic transformation of the Bacillus subtilis genospecies.
    J Bacteriol. 1972 Sep;111(3):705-16 PMID: 4626541
  14. Size of DNA determined by viscoelastic measurements: results on bacteriophages, Bacillus subtilis and Escherichia coli.
    J Mol Biol. 1972 Dec 30;72(3):779-800 PMID: 4633907
  15. Construction of biologically functional bacterial plasmids in vitro.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
  16. Plasmid in Bacillus pumilus and the enhanced sporulation of plasmid-negative variants.
    J Bacteriol. 1973 Jul;115(1):291-8 PMID: 4717517
  17. Identification of Bacillus subtilis NRRL B-3275 as a strain of Bacillus pumilus.
    J Bacteriol. 1969 Nov;100(2):658-61 PMID: 4982197
  18. Cotransduction and cotransformation of genetic markers in Bacillus subtilis and Bacillus licheniformis.
    J Bacteriol. 1969 Nov;100(2):1027-36 PMID: 4982666
  19. Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies.
    Proc Natl Acad Sci U S A. 1965 Aug;54(2):491-8 PMID: 4956287
  20. [The group of genes regulating the biosynthesis of tryptophan in Bacillus subtilis].
    C R Acad Sci Hebd Seances Acad Sci D. 1967 Jul 3;265(1):93-6 PMID: 4963644
  21. Interspecies transformation between Bacillus subtilis and Bacillus licheniformis.
    Biochem Biophys Res Commun. 1966 May 25;23(4):543-8 PMID: 4960110
  22. Development of competence in the Bacillus subtilis transformation system.
    J Bacteriol. 1967 Sep;94(3):562-70 PMID: 4962301
  23. Low-frequency, pbsi-mediated plasmid transduction in Bacillus pumilus.
    J Bacteriol. 1976 Aug;127(2):829-31 PMID: 821920
  24. Bacillus pumilus plasmid pPL10: properties and insertion into Bacillus subtilis 168 by transformation.
    J Bacteriol. 1976 Aug;127(2):817-28 PMID: 821919
  25. Plasmid deoxyribonucleic acid in Bacillus subtilis and Bacillus pumilus.
    J Bacteriol. 1975 Oct;124(1):484-90 PMID: 809424
  26. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  27. Biochemical construction and selection of hybrid plasmids containing specific segments of the Escherichia coli genome.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4361-5 PMID: 1105581
  28. Genetic analysis in Bacillus pumilus by PBSI-mediated transduction.
    J Bacteriol. 1970 Feb;101(2):603-8 PMID: 5413829
  29. Sequential replication of the chromosome of Bacillus licheniformis.
    J Bacteriol. 1968 Jun;95(6):2062-9 PMID: 5669889
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.3.1423
Published
1978-03-00
Pages
1423-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411484
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.3.1423 PMC Full Text View journal details
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