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PMID: 107388 Published · ppublish English Journal Article

High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.

Molecular & general genetics : MGG ·Vol. 168 ·No. 1 ·1979-01-05 ·Pages 111-5

Chang S, Cohen SN

Abstract

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) transformants per microgram of supercoiled DNA. Plasmids constructed by in vitro ligation or endonuclease-generated fragments of linear plasmid DNA can also transform PEG-treated protoplasts, but at a lower frequency.

MeSH Terms
Bacillus subtilis/genetics Chloramphenicol/pharmacology DNA, Circular/genetics Kanamycin/pharmacology Kinetics Protoplasts/metabolism R Factors Transformation, Bacterial
Chemicals
DNA, Circular Kanamycin Chloramphenicol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chang S
Cohen S N
References (21)
21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267940
Published
1979-01-05
Pages
111-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267940
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