DNA cloning in Bacillus subtilis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 3 ·1978-03-00 ·Pages 1433-6

Ehrlich SD

Abstract

A plasmid pC194, encoding resistance to chloramphenicol, can serve as a cloning vector in Bacillus subtilis 168 for other HindIII-cleaved DNA segments. Replicons constructed by linking pC194 to several Escherichia coli plasmids can be used to introduce and compare the expression of the same genes in these two bacterial hosts.

MeSH Terms

Bacillus subtilis/genetics Chloramphenicol DNA Restriction Enzymes DNA, Bacterial/genetics DNA, Recombinant Drug Resistance, Microbial Escherichia coli/genetics Plasmids Staphylococcus aureus/genetics Tetracycline/pharmacology

Chemicals

DNA, Bacterial DNA, Recombinant Chloramphenicol DNA Restriction Enzymes Tetracycline

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Ehrlich S D

References (14)

14 references, click to expand

Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4145-9 PMID: 825858
Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680-2 PMID: 404641
Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.
J Bacteriol. 1977 Jul;131(1):374-7 PMID: 406259
Isolation and characterization of four plasmids from Bacillus subtilis.
J Bacteriol. 1977 Mar;129(3):1487-94 PMID: 403179
Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae.
J Bacteriol. 1977 Aug;131(2):557-63 PMID: 407214
Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4.
Mol Gen Genet. 1976 Sep 23;147(3):315-23 PMID: 787766
Enhancement of transfecting activity of bacteriophage P22 DNA upon exonucleolytic erosion.
J Mol Biol. 1976 Aug 25;105(4):587-602 PMID: 787541
Construction of biologically functional bacterial plasmids in vitro.
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1030-4 PMID: 4598290
Spontaneous release of DNA in sequential genetic order by Bacillus subtilis.
J Mol Biol. 1969 Oct 14;45(1):137-52 PMID: 4981003
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
Bacillus pumilus plasmid pPL10: properties and insertion into Bacillus subtilis 168 by transformation.
J Bacteriol. 1976 Aug;127(2):817-28 PMID: 821919
Electrophoretic separation of Bacillus subtilis genes.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2207-11 PMID: 806078
Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b.
J Bacteriol. 1976 Apr;126(1):439-46 PMID: 770429

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.3.1433

Published

1978-03-00

Pages

1433-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC411486

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.3.1433 PMC Full Text View journal details

Analysis Services

DNA cloning in Bacillus subtilis.

Contact

WeChat Official Account