Home LiteratureArticle Details
PMID: 407214 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae.

Journal of bacteriology ·Vol. 131 ·No. 2 ·1977-08-00 ·Pages 557-63

Roberts M, Elwell LP, Falkow S

Abstract

The molecular nature of two distinct gonococcal R plasmids, 4.4 X 10(6) and 3.2 X 10(6) daltons, encoding beta-lactamase activity were examined. Both plasmids contained about 40% of the transposable ampicillin resistance sequence Tn2. Deoxyribonucleic acid-deoxyribonucleic acid polynucleotide sequence studies have shown that the two gonococcal plasmids share about 70% of their sequences and are closely related to RSF0885, a 4.1 X 10(6)-dalton plasmid found in a beta-lactamase-producing strain of Haemophilus influenzae. All three of these R plasmids possess a guanine-plus-cytosine content of 0.40 to 0.41 mol fraction and are present as multicopy gene pools in their bacterial hosts.

MeSH Terms
Base Sequence Cytosine/analysis DNA, Bacterial/analysis DNA, Circular/analysis Drug Resistance, Microbial Escherichia coli Extrachromosomal Inheritance Guanine/analysis Haemophilus influenzae Neisseria gonorrhoeae/enzymology Nucleic Acid Hybridization Penicillinase/biosynthesis Plasmids R Factors Transformation, Genetic
Chemicals
DNA, Bacterial DNA, Circular Guanine Cytosine Penicillinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roberts M
Elwell L P
Falkow S
References (28)
28 references, click to expand
  1. Transposable genetic elements and plasmid evolution.
    Nature. 1976 Oct 28;263(5580):731-8 PMID: 792710
  2. Structural evolution of bacterial plasmids: role of translocating genetic elements and DNA sequence insertions.
    Fed Proc. 1976 Jul;35(9):2031-6 PMID: 776704
  3. Conjugal transfer of R plasmids in Neisseria gonorrhoeae.
    Nature. 1977 Apr 14;266(5603):630-1 PMID: 404570
  4. Plasmid-mediated beta-lactamase production in Neisseria gonorrhoeae.
    Antimicrob Agents Chemother. 1977 Mar;11(3):528-33 PMID: 404964
  5. Characterization of an R-plasmid associated with ampicillin resistance in Shigella dysenteriae type 1 isolated from epidemics.
    Antimicrob Agents Chemother. 1977 Mar;11(3):553-8 PMID: 324394
  6. Plasmid-mediated chloramphenicol resistance in Haemophilus influenzae.
    Antimicrob Agents Chemother. 1977 Mar;11(3):383-7 PMID: 324388
  7. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.
    Antimicrob Agents Chemother. 1972 Apr;1(4):283-8 PMID: 4208895
  8. Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae.
    Infect Immun. 1974 Oct;10(4):712-7 PMID: 4214778
  9. Penicillinase-producing Neisseria gonorrhoeae.
    Lancet. 1976 Sep 25;2(7987):657-8 PMID: 60519
  10. Beta-lactamase-producing, penicillin-resistant gonococcus.
    Lancet. 1976 Sep 25;2(7987):656-7 PMID: 60518
  11. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing.
    J Infect Dis. 1973 Aug;128(2):178-94 PMID: 4198720
  12. Physical evidence of a plasmid in Neisseria gonorrhoeae.
    J Infect Dis. 1973 Feb;127(2):197-200 PMID: 4632044
  13. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  14. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  15. Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium.
    Nature. 1965 Nov 27;208(5013):843-9 PMID: 5331112
  16. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.
    J Bacteriol. 1976 Sep;127(3):1529-37 PMID: 821935
  17. Plasmid deoxyribonucleic acid in clinical isolates of Neisseria gonorrhoeae.
    J Bacteriol. 1975 Jun;122(3):1293-300 PMID: 807554
  18. Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.
    Infect Immun. 1975 May;11(5):1141-6 PMID: 804444
  19. [Tetracycline resistance in Haemophilus influenzae is plasmid-linked].
    C R Acad Sci Hebd Seances Acad Sci D. 1975 Mar 10;280(10):1321-3 PMID: 807346
  20. Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids.
    J Bacteriol. 1975 Jan;121(1):234-8 PMID: 1090570
  21. Origin of the TEM-beta-lactamase gene found on plasmids.
    J Bacteriol. 1975 Apr;122(1):250-6 PMID: 1091625
  22. Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3623-7 PMID: 1103150
  23. A transferable kanamycin resistance plasmid isolated from Haemophilus influenzae.
    Ann Microbiol (Paris). 1975 Apr;126(3):397-9 PMID: 1103677
  24. Plasmid-linked ampicillin resistance in haempohilus influenza type b.
    Infect Immun. 1975 Aug;12(2):404-10 PMID: 1080132
  25. Rapid detection of ampicillin-resistant Haemophilus influenzae and their susceptibility to sixteen antibiotics.
    Antimicrob Agents Chemother. 1975 Jul;8(1):91-4 PMID: 1080656
  26. Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b.
    J Bacteriol. 1976 Apr;126(1):439-46 PMID: 770429
  27. Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Eschericha coli.
    J Bacteriol. 1976 Apr;126(1):1-6 PMID: 770415
  28. Penicillinase-producing Gonococci in Liverpool.
    Lancet. 1976 Dec 25;2(8000):1379-82 PMID: 63850
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.131.2.557-563.1977
Published
1977-08-00
Pages
557-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235464
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.131.2.557-563.1977 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2