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PMID: 404964 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasmid-mediated beta-lactamase production in Neisseria gonorrhoeae.

Antimicrobial agents and chemotherapy ·Vol. 11 ·No. 3 ·1977-03-00 ·Pages 528-33

Elwell LP, Roberts M, Mayer LW, Falkow S

Abstract

Several beta-lactamase-producing, penicillin-resistant strains of Neisseria gonorrhoeae were examined for R plasmids. Penicillin-resistant strains isolated from men returning from the Far East and their contacts contained a 4.4 x 10(6)-dalton plasmid in common. Transformation studies and the isolation of a spontaneous penicillin-susceptible segregant showed that the structural gene for beta-lactamase was part of the 4.4 x 10(6)-dalton plasmid. An additional penicillin-resistant gonococcal strain isolated in London was found to harbor a 3.2 x 10(6)-dalton R plasmid. Deoxyribonucleic acid (DNA)-DNA duplex studies revealed that the penicillin-resistant gonococcal isolates contained a significant portion (about 40%) of the transposable DNA sequence, TnA, which includes the beta-lactamase gene commonly found on R plasmids of the Enterobacteriaceae and Haemophilus influenzae.

MeSH Terms
DNA, Bacterial/analysis Drug Resistance, Microbial Electrophoresis, Agar Gel Extrachromosomal Inheritance Genes Neisseria gonorrhoeae/analysis,enzymology Nucleic Acid Hybridization Penicillin Resistance Penicillinase/biosynthesis Plasmids R Factors
Chemicals
DNA, Bacterial Penicillinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Elwell L P
Roberts M
Mayer L W
Falkow S
References (25)
25 references, click to expand
  1. Transposable genetic elements and plasmid evolution.
    Nature. 1976 Oct 28;263(5580):731-8 PMID: 792710
  2. Transposition of a plasmid deoxyribonucleic acid sequence that mediates ampicillin resistance: independence from host rec functions and orientation of insertion.
    J Bacteriol. 1976 Oct;128(1):425-34 PMID: 789346
  3. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.
    Antimicrob Agents Chemother. 1972 Apr;1(4):283-8 PMID: 4208895
  4. Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae.
    Infect Immun. 1974 Oct;10(4):712-7 PMID: 4214778
  5. Penicillinase-producing Neisseria gonorrhoeae.
    Lancet. 1976 Sep 25;2(7987):657-8 PMID: 60519
  6. Beta-lactamase-producing, penicillin-resistant gonococcus.
    Lancet. 1976 Sep 25;2(7987):656-7 PMID: 60518
  7. Transposition of ampicillin resistance from RP4 to other replicons.
    Mol Gen Genet. 1974;132(1):31-40 PMID: 4609125
  8. Physical evidence of a plasmid in Neisseria gonorrhoeae.
    J Infect Dis. 1973 Feb;127(2):197-200 PMID: 4632044
  9. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  10. Haemophilus influenzae type B resistant to ampicillin. A report of two cases.
    JAMA. 1974 Jul 15;229(3):298-301 PMID: 4546094
  11. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  12. Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes.
    J Bacteriol. 1973 Sep;115(3):904-11 PMID: 4728274
  13. The ecology of transferable drug resistance in the enterobacteria.
    Annu Rev Microbiol. 1968;22:131-80 PMID: 4879515
  14. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.
    J Bacteriol. 1976 Sep;127(3):1529-37 PMID: 821935
  15. Plasmid deoxyribonucleic acid in clinical isolates of Neisseria gonorrhoeae.
    J Bacteriol. 1975 Jun;122(3):1293-300 PMID: 807554
  16. Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.
    Infect Immun. 1975 May;11(5):1141-6 PMID: 804444
  17. [Tetracycline resistance in Haemophilus influenzae is plasmid-linked].
    C R Acad Sci Hebd Seances Acad Sci D. 1975 Mar 10;280(10):1321-3 PMID: 807346
  18. Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids.
    J Bacteriol. 1975 Jan;121(1):234-8 PMID: 1090570
  19. Origin of the TEM-beta-lactamase gene found on plasmids.
    J Bacteriol. 1975 Apr;122(1):250-6 PMID: 1091625
  20. Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3623-7 PMID: 1103150
  21. A transferable kanamycin resistance plasmid isolated from Haemophilus influenzae.
    Ann Microbiol (Paris). 1975 Apr;126(3):397-9 PMID: 1103677
  22. Plasmid-linked ampicillin resistance in haempohilus influenza type b.
    Infect Immun. 1975 Aug;12(2):404-10 PMID: 1080132
  23. Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b.
    J Bacteriol. 1976 Apr;126(1):439-46 PMID: 770429
  24. Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Eschericha coli.
    J Bacteriol. 1976 Apr;126(1):1-6 PMID: 770415
  25. Structural evolution of bacterial plasmids: role of translocating genetic elements and DNA sequence insertions.
    Fed Proc. 1976 Jul;35(9):2031-6 PMID: 776704
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.11.3.528
Published
1977-03-00
Pages
528-33
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC352016
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.11.3.528 PMC Full Text View journal details
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