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PMID: 1367370 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain.

Applied microbiology and biotechnology ·Vol. 35 ·No. 2 ·1991-05-00 ·Pages 247-52

Henschke RB, Henschke EJ, Schmidt FR

Abstract

A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using different monitoring methods, including the polymerase-chain reaction, to amplify and detect the specific rDNA. Depending on nutritional status, both the GEM and its rDNA had disappeared within 16 (amended soil) or 28 days (non-amended soil) with a limit of detection of 5 cells/g soil and 20 fg DNA/g soil.

MeSH Terms

Base Sequence Cloning, Molecular DNA, Bacterial/isolation & purification Ecology Escherichia coli/genetics Genetic Markers Models, Biological Molecular Sequence Data Polymerase Chain Reaction Soil Microbiology Transfection

Chemicals

DNA, Bacterial Genetic Markers

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Henschke R B

Bundesforschungsanstalt für Landwirtschaft (FAL), Institut für Bodenbiologie, Braunschweig, Federal Republic of Germany.

Henschke E J

Schmidt F R

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12 references, click to expand

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Article Info

Journal

Applied microbiology and biotechnology

Abbr.

Appl Microbiol Biotechnol

ISSN

0175-7598

DOI

10.1007/BF00184696

Published

1991-05-00

Pages

247-52

Language

English

Region

Germany

NLM ID

8406612

External Links

PubMed source DOI: 10.1007/BF00184696 View journal details

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