Molecular cloning in Lactobacillus helveticus by plasmid pSA3::pVA797 co-integrate formation and conjugal transfer.
A gene encoding beta-glucanase activity from Bacillus amyloliquefaciens was subcloned in both orientations into plasmid shuttle vector pSA3. In only one orientation could a co-integrate be generated with the conjugative plasmid pVA797. The plasmid co-integrate was conjugated into Lactobacillus helveticus strain CNRZ450, where it was stably maintained without antibiotic selection and exhibited beta-glucanase activity. This method of introducing cloned DNA into thermophilic lactobacilli will facilitate the study of heterologous gene expression in non-transformable species.
16 references, click to expand
-
Physical characterisation of the replication region of the Streptococcus faecalis plasmid pAM beta 1.Gene. 1990 Mar 1;87(1):79-90 PMID: 2110101
-
Cloning and expression of the beta-D-phosphogalactoside galactohydrolase gene of Lactobacillus casei in Escherichia coli K-12.J Bacteriol. 1982 Dec;152(3):1138-46 PMID: 6292163
-
Applications for biotechnology: present and future improvements in lactic acid bacteria.FEMS Microbiol Rev. 1990 Sep;7(1-2):3-14 PMID: 2271224
-
Transfection of Lactobacillus bulgaricus protoplasts by bacteriophage DNA.Appl Environ Microbiol. 1988 Dec;54(12):3014-8 PMID: 3066292
-
Molecular cloning and expression of a Bacillus subtilis beta-glucanase gene in Escherichia coli.Gene. 1983 Aug;23(2):211-9 PMID: 6311687
-
Simple and rapid method for isolating large plasmid DNA from lactic streptococci.Appl Environ Microbiol. 1983 Sep;46(3):549-52 PMID: 6416164
-
Streptococcus-Escherichia coli shuttle vector pSA3 and its use in the cloning of streptococcal genes.Appl Environ Microbiol. 1985 Jan;49(1):115-9 PMID: 3883896
-
Conjugal transfer of plasmid pIP501 from Lactococcus lactis to Lactobacillus delbrückii subsp. bulgaricus and Lactobacillus helveticus.FEMS Microbiol Lett. 1989 Jul 15;51(1):149-52 PMID: 2506107
-
Conjugal transfer of group B streptococcal plasmids and comobilization of Escherichia coli-Streptococcus shuttle plasmids to Lactobacillus plantarum.Appl Environ Microbiol. 1988 Mar;54(3):824-6 PMID: 3132101
-
Streptococcal R plasmid pIP501: endonuclease site map, resistance determinant location, and construction of novel derivatives.J Bacteriol. 1983 Jun;154(3):1347-55 PMID: 6304011
-
Region of the streptococcal plasmid pMV158 required for conjugative mobilization.J Bacteriol. 1989 Sep;171(9):4778-84 PMID: 2768188
-
Conjugal mobilization of streptococcal plasmid pMV158 between strains of Lactococcus lactis subsp. lactis.J Bacteriol. 1990 Jan;172(1):47-52 PMID: 2104609
-
Conjugative mobilization as an alternative vector delivery system for lactic streptococci.Appl Environ Microbiol. 1987 Oct;53(10):2405-13 PMID: 3122654
-
Integration and expression of alpha-amylase and endoglucanase genes in the Lactobacillus plantarum chromosome.Appl Environ Microbiol. 1989 Sep;55(9):2130-7 PMID: 2679379
-
Evidence for the conjugal transfer of the broad host range plasmid pIP501 into strains of Lactobacillus helveticus.J Appl Bacteriol. 1988 Oct;65(4):309-19 PMID: 3146566
-
Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum.Appl Environ Microbiol. 1989 Aug;55(8):2095-7 PMID: 2782880
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