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PMID: 13691706 Published · ppublish English Journal Article

The replication of DNA III. Changes in the number of strands in E. coli DNA during its replication cycle.

Biophysical journal ·Vol. 1 ·1961-03-00 ·Pages 337-51

CAVALIERI LF, ROSENBERG BH

Abstract

DNA has been isolated from synchronized cultures of E. coli 15(T)- at various times. At first the DNA was four-stranded (and indistinguishable in all respects from log phase E. coli DNA), but at the start of DNA synthesis the DNA was found to have halved its molecular weight and to have become two-stranded. This sample had all the properties of undenatured, double-helical DNA, and behaved in all respects like DNA from non-proliferating sources. The replication cycle of the DNA molecule has thus been shown to consist of an alternation between the four- and two-stranded forms, the latter being the conserved unit. The evidence provided by the three papers of this series with respect to DNA and chromosomal structure and replication is discussed and summarized.

Keywords
DESOXYRIBONUCLEIC ACID/chemistry ESCHERICHIA COLI/metabolism
MeSH Terms
DNA/chemistry DNA Replication Escherichia coli/metabolism
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
CAVALIERI L F
ROSENBERG B H
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30 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/s0006-3495(61)86893-8
Published
1961-03-00
Pages
337-51
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1366352
Subset
OM
External Links
PubMed source DOI: 10.1016/s0006-3495(61)86893-8 PMC Full Text View journal details
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