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PMID: 1371205 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Changes in distribution, morphology, and tumor necrosis factor-alpha secretion of alveolar macrophage subpopulations during the development of bleomycin-induced pulmonary fibrosis.

The American journal of pathology ·Vol. 140 ·No. 2 ·1992-02-00 ·Pages 503-12

Everson MP, Chandler DB

Abstract

Previous studies indicate that heterogeneous alveolar macrophages (AM) play a pivotal role in events associated with bleomycin-induced pulmonary fibrosis. A critical role has been suggested for tumor necrosis factor-alpha (TNF-alpha), a product of activated macrophages, in this fibrotic process. The present study examined whether the characteristics and function (TNF-alpha secretion) of rat AM subpopulations were altered during the development of bleomycin-induced fibrosis. After intratracheal bleomycin treatment, AM were separated into 18 density-defined subpopulations. Bleomycin treatment altered the distribution and morphology of AM subpopulations of densities 1.017 to 1.061 g/ml at all time points studied (4, 14, and 28 days). Subpopulations of densities 1.090 to 1.141 g/ml were affected only at 4 days after bleomycin treatment. Tumor necrosis factor-alpha secretion increased with time in 14- and 28-day samples of bleomycin-treated rats, particularly in subpopulations of densities 1.075 to 1.097 g/ml. These data indicate that alterations in the distribution, morphology, and function of AM subpopulations accompany the development of bleomycin-induced pulmonary fibrosis. When coupled with previous studies suggesting that TNF-alpha plays a role in the fibrotic process in this disease model, these data indicate that AM of densities 1.075 to 1.097 g/ml are responsible for the production of TNF-alpha associated with bleomycin-induced pulmonary fibrosis.

MeSH Terms
Animals Bleomycin Cell Division Cell Separation Macrophages, Alveolar/metabolism,pathology Male Pulmonary Fibrosis/chemically induced,metabolism,pathology Rats Rats, Inbred F344 Tumor Necrosis Factor-alpha/metabolism
Chemicals
Tumor Necrosis Factor-alpha Bleomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Everson M P
Department of Pathology, University of Alabama, Birmingham.
Chandler D B
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39 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1992-02-00
Pages
503-12
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1886424
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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