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PMID: 1371248 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Critical role of a common transcription factor, IRF-1, in the regulation of IFN-beta and IFN-inducible genes.

The EMBO journal ·Vol. 11 ·No. 1 ·1992-01-00 ·Pages 185-93

Reis LF, Harada H, Wolchok JD, Taniguchi T, Vilcek J

Abstract

Interferon regulatory factor 1 (IRF-1) is a protein that binds to cis-elements within the promoter of interferon (IFN)-beta and some IFN-inducible genes. We used a human fibroblast line, GM-637, to generate stable transfectants constitutively expressing IRF-1 mRNA in either the sense or antisense orientation. Upon induction with poly-(I).poly(C) or Newcastle disease virus, cells expressing sense IRF-1 mRNA produced significantly higher levels of IFN-beta mRNA and protein than control cells, whereas cells expressing antisense IRF-1 mRNA produced little or no IFN-beta mRNA and protein. Furthermore, clear differences were seen among the transfectants in the level of expression of two IFN-induced genes (2'-5'-oligoadenylate synthetase and class I HLA). Our data show that IRF-1 is essential for the induced expression of the IFN-beta gene. The results also indicate an important role of IRF-1 in the expression of IFN-inducible genes and suggest a role for IRF-1 in many other cytokine actions.

MeSH Terms
2',5'-Oligoadenylate Synthetase/metabolism Cell Line, Transformed DNA-Binding Proteins/genetics Fibroblasts/cytology,drug effects Gene Expression Regulation, Neoplastic/drug effects Humans Interferon Regulatory Factor-1 Interferons/pharmacology Major Histocompatibility Complex/genetics Phosphoproteins/genetics RNA RNA, Antisense Skin/cytology Transcription Factors/genetics Transfection
Chemicals
DNA-Binding Proteins IRF1 protein, human Interferon Regulatory Factor-1 Phosphoproteins RNA, Antisense RNA, recombinant Transcription Factors RNA Interferons 2',5'-Oligoadenylate Synthetase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reis L F
Department of Microbiology, New York University Medical Center, NY 10016.
Harada H
Wolchok J D
Taniguchi T
Vilcek J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-01-00
Pages
185-93
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556439
Subset
IM
Grants
NCI NIH HHS · 1-R35-CA49731 · United States
NIGMS NIH HHS · 5-T32-GM07308 · United States
NCI NIH HHS · CA16087-14 · United States
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PubMed source PMC Full Text View journal details
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