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PMID: 2183014 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of eight tumor necrosis factor-induced gene sequences from human fibroblasts.

Molecular and cellular biology ·Vol. 10 ·No. 5 ·1990-05-00 ·Pages 1982-8

Lee TH, Lee GW, Ziff EB, Vilcek J

Abstract

A lambda cDNA library was prepared from polyadenylated RNA isolated from quiescent human diploid FS-4 fibroblasts stimulated with tumor necrosis factor for 3 h. Differential screening was used to isolate cDNA sequences that are stimulated by tumor necrosis factor. Eight distinct tumor necrosis factor-stimulated gene sequences (designated TSG-1, -6, -8, -12, -14, -21, -27, and -37) were partially sequenced and compared with known sequences from GenBank. TSG-1 was identical to the gene for interleukin-8. TSG-8 corresponded to the gene for monocyte chemotactic and activating factor. TSG-21 and -27 were identical to the genes for collagenase and stromelysin, respectively. The other four sequences showed no homologies with known genes. Patterns of induction of mRNAs corresponding to the eight cloned cDNAs by various cytokines, growth factors, and activators of second messenger pathways were analyzed in FS-4 cells.

MeSH Terms
Base Sequence Biological Factors/pharmacology Chemotactic Factors/genetics Cloning, Molecular Cytokines DNA/genetics Fibroblasts Gene Expression Regulation/drug effects Gene Library Humans Interleukin-8 Interleukins/genetics Kinetics Molecular Sequence Data RNA, Messenger/genetics Tumor Necrosis Factor-alpha/pharmacology
Chemicals
Biological Factors Chemotactic Factors Cytokines Interleukin-8 Interleukins RNA, Messenger Tumor Necrosis Factor-alpha DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee T H
Department of Microbiology, New York University Medical Center, New York 10016.
Lee G W
Ziff E B
Vilcek J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.5.1982-1988.1990
Published
1990-05-00
Pages
1982-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360544
Subset
IM
Grants
NCI NIH HHS · P01-CA16239 · United States
NIAID NIH HHS · R01-AI12948 · United States
NCI NIH HHS · R01-CA4304 · United States
Databases
GENBANK
M31163, M31164, M31165, M31166
External Links
PubMed source DOI: 10.1128/mcb.10.5.1982-1988.1990 PMC Full Text View journal details
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